Mouse Von Hippel- Lindau disease tumor suppressor, Vhl ELISA KIT

Product Name:Mouse Von Hippel- Lindau disease tumor suppressor, Vhl ELISA KIT
Packing:96T

Catalog No.:ELI-06081m

Gene Name:Mouse Vhl

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Vhl

Alternative Name:Mouse Von Hippel-Lindau disease tumor suppressor, Vhl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Von Hippel- Lindau disease tumor suppressor, Vhl ELISA KIT allows for the in vitro quantitative determination of Mouse Vhl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Von Hippel- Lindau disease tumor suppressor, Vhl ELISA KIT has been pre-coated with an Mouse Von Hippel-Lindau disease tumor suppressor, Vhl antibody specific to Mouse Vhl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Vhl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Vhl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Vhl in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!