Mouse Zinc finger protein GLI2, Gli2 ELISA KIT

Product Name:Mouse Zinc finger protein GLI2, Gli2 ELISA KIT
Packing:96T

Catalog No.:ELI-48799m

Gene Name:Mouse Gli2

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Gli2

Alternative Name:Mouse Zinc finger protein GLI2, Gli2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Zinc finger protein GLI2, Gli2 ELISA KIT allows for the in vitro quantitative determination of Mouse Gli2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Zinc finger protein GLI2, Gli2 ELISA KIT has been pre-coated with an Mouse Zinc finger protein GLI2, Gli2 antibody specific to Mouse Gli2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gli2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gli2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gli2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!