ATHDOC (Allotetrahydrodeoxycorticosterone) ELISA KIT

$796.00

  • Model: CELI-E0150Ge
  • 4 Units in Stock
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ATHDOC (Allotetrahydrodeoxycorticosterone) ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Shorten Name  THDOC

Target Name  allotetrahydrodeoxycorticosterone

Alternative Name

allotetrahydrodeoxycorticosterone ELISA KIT, Tetrahydrodeoxycorticosterone ELISA KIT, THDOC ELISA KIT, 3α,21-dihydroxy-5α-pregnan-20-one ELISA KIT, ATHDOC ELISA KIT

 

Allotetrahydrodeoxycorticosterone ELISA KIT Detect Range  1.56-100NG/ML

 

Intended use 

Allotetrahydrodeoxycorticosterone ELISA KIT allows for the in vitro quantitative determination of Allotetrahydrodeoxycorticosterone concentrations in serum, plasma, tissue homogenates,cell culture supernates or other biological fluids.

 

Allotetrahydrodeoxycorticosterone ELISA KIT Content List

Reagent  Quantity

Assay plate      1      

Standard  2      

Sample Diluent         1 × 20mL 

Assay Diluent A        1 × 10mL 

Assay Diluent B        1 × 10mL 

Detection Reagent A       1 × 60μL  

Detection Reagent B       1 × 120μL         

Wash Buffer(25 x concentrate)        1 × 30mL 

Substrate          1 × 10mL 

Stop Solution    1 × 10mL 

Plate sealer      5      

 

ATHDOC (Allotetrahydrodeoxycorticosterone) ELISA KIT Description

Allotetrahydrodeoxycorticosterone ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter  plate provided in Allotetrahydrodeoxycorticosterone ELISA KIT has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each  well.  The  enzyme-substrate  reaction is  terminated by the addition of a sulphuric acid solution and the color change is measured spectro- photometrically at  a  wavelength  of  450  nm ± 2 nm.  The  concentration of target  antigen  in  the  samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Caution:
Product is for research use only!

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