Equine Insulin like growth factor I, IGF1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Insulin-like growth factor I,IGF-I,Somatomedin, IGF1,IGF-1,IGF-I; IGF1A; IGFI; IGF-IA; IGF-IB; MGF; insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C; insulin-like growth factor 1

Search name

Equine IGF1 ELISA KIT,Equine IGF-I ELISA KIT ,Equine Insulin-like growth factor I ELISA KIT ,Equine Somatomedin ELISA KIT ,Equine  IGF1 ELISA KIT ,Equine IGF-1 ELISA KIT ,Equine IGF-I ELISA KIT ,Equine IGF1A ELISA KIT ,Equine IGFI ELISA KIT ,Equine IGF-IA ELISA KIT ,Equine IGF-IB ELISA KIT ,Equine MGF ELISA KIT ,Equine insulin-like growth factor 1 ELISA KIT ,Equine insulin-like growth factor I ELISA KIT ,Equine insulin-like growth factor IA ELISA KIT ,Equine insulin-like growth factor IB ELISA KIT ,Equine mechano growth factor ELISA KIT ,Equine somatomedin-C ELISA KIT ,Equine insulin-like growth factor 1 ELISA KIT

Intended use

Equine IGF1 ELISA KIT allows for the in vitro quantitative determination of equine insulin-like growth factor i,IGF-I concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Equine IGF1 ELISA KIT has been pre-coated with an antibody specific to IGF-I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IGF-I and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGF-I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGF-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Equine IGF1 ELISA KIT Range 3.21-200ng/ml

Equine IGF1 ELISA KIT PDF manual download

Order IGF1 ELISA KIT by email:[email protected]