Fish Interleukin- 1 beta, IL1b ELISA Kit
96 Tests
FOR RESEARCH USE ONLY!

Synonyms
Interleukin-1 beta(IL-1 beta),;Catabolin,; interleukin-1 beta; IL-1beta; IL1beta; IL-1 beta; interleukin 1, beta

Search name
Fish IL1b ELISA Kit,Fish Interleukin-1 beta ELISA Kit, Fish IL-1b ELISA Kit, Fish Interleukin-1b ELISA Kit, Fish IL-1beta ELISA Kit, Fish Catabolin ELISA KIT,Fish IL1F2 ELISA KIT, Interleukin-1 beta ELISA Kit, IL-1b ELISA Kit, Interleukin-1b ELISA Kit, IL-1beta ELISA Kit, Catabolin ELISA KIT,IL1F2 ELISA KIT

Intended use
Fish IL1b ELISA Kit allows for the in vitro quantitative determination of fish IL-1 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

Range 31.2-2000 pg/ml

PDF manual download

Order it by email:[email protected]