General Platelet- activating factor, PAF ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Platelet-activating factor,; PAFA,PAF, PAF-acether , AGEPC,acetyl-glyceryl-ether-phosphorylcholine

Search name
General Platelet- activating factor ELISA Kit, General PAF ELISA Kit, General PAFA ELISA Kit, General PAF-acether ELISA Kit,
General AGEPC ELISA Kit, General acetyl-glyceryl-ether-phosphorylcholine ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of platelet-activating factor,Platelet-activating factor
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Platelet-activating factor, During the reaction, Platelet-activating factor in the sample or standard
competes with a fixed amount of biotin-labeled Platelet-activating factor for sites on a pre-coated Monoclonal antibody specific to
Platelet-activating factor. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Platelet-activating factor in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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