General Vitamin D3, VD3 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vitamin D3,Cholecalciferol,

Search name
General Vitamin D3 ELISA Kit, General VD3 ELISA Kit, General Cholecalciferol ELISA Kit, Vitamin D3 ELISA Kit, VD3 ELISA Kit,
Cholecalciferol ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general vitamin d3,Vitamin D3 concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Vitamin D3, During the reaction, Vitamin D3 in the sample or standard competes with a fixed
amount of biotin-labeled Vitamin D3 for sites on a pre-coated Monoclonal antibody specific to Vitamin D3. Excess conjugate and unbound
sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration
of Vitamin D3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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