Goat Interleukin-1, IL1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-1 beta(IL-1 beta),; Interleukin 1beta,IL-1b,IL-1,IL1, Lymphocyte proliferation-potentiating factor

Search name
Goat Interleukin 1 ELISA Kit, Goat IL-1 ELISA Kit, Goat Interleukin- 1 beta ELISA Kit, Goat IL- 1 beta ELISA Kit, Interleukin 1
ELISA Kit, IL-1 ELISA Kit, Lymphocyte proliferation-potentiating factor ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of goat interleukin-1 beta,IL-1 beta concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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