Plant Gibberellic Acid, GA ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Gibberellic Acid; GA, Gibberellin A3, GA, GA3

Search name
Plant Gibberellic Acid ELISA Kit, Plant GA ELISA Kit, Plant GA3 ELISA Kit, Plant Gibberellin A3 ELISA Kit

Intended use
Plant GA ELISA Kit allows for the in vitro quantitative determination of plant Gibberellic Acid,GA concentrations in serum, Plasma ,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided Plant GA ELISA Kit has been pre-coated with an antibody specific to GA. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GA and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain GA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GA in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

Plant GA ELISA Kit PDF manual download