Salmon Insulin like growth factor I, IGF1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

IGF1,Insulin-like growth factor I,IGF-I,Somatomedin, IGF-1,IGF-I; IGF1A; IGFI; IGF-IA; IGF-IB; MGF; insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C; insulin-like growth factor 1

Search name

Salmon IGF1 ELISA KIT ,Salmon Insulin-like growth factor I ELISA KIT ,Salmon IGF-I ELISA KIT ,Salmon Somatomedin ELISA KIT ,Salmon IGF-1 ELISA KIT ,Salmon IGF-I ELISA KIT ,Salmon IGF1A ELISA KIT ,Salmon IGFI ELISA KIT ,Salmon IGF-IA ELISA KIT ,Salmon IGF-IB ELISA KIT ,Salmon MGF ELISA KIT ,Salmon insulin-like growth factor 1 ELISA KIT ,Salmon insulin-like growth factor I ELISA KIT ,Salmon insulin-like growth factor IA ELISA KIT ,Salmon insulin-like growth factor IB ELISA KIT ,Salmon mechano growth factor ELISA KIT ,Salmon somatomedin-C ELISA KIT ,Salmon insulin-like growth factor 1 ELISA KIT

Intended use

Salmon IGF1 ELISA KIT allows for the in vitro quantitative determination of salmon insulin-like growth factor i,IGF-I concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided Salmon IGF1 ELISA KIT has been pre-coated with an antibody specific to IGF-I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IGF-I and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGF-I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGF-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Salmon IGF1 ELISA KIT Range 0.31-20ng/ml

Salmon IGF1 ELISA KIT PDF manual download

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