Human 4-HNE ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Shorten Name 4-HNE
Target Name 4-Hydroxynonenal
Alternative Name
Human 4-HNE ELISA KIT ,Human 4-Hydroxynonenal ELISA KIT ,Human 4-hydroxy-2-nonenal ELISA KIT ,Human HNE ELISA KIT ,Human 4-Hydroxynon-2-enal ELISA KIT
Human 4-HNE ELISA KIT Detect Range 0.62-40ng/ml
Intended use
Human 4-HNE ELISA KIT allows for the in vitro quantitative determination of 4-HNE concentrations in serum, plasma, tissue homogenates,cell culture supernates or other biological fluids.
Human 4-HNE ELISA KIT Content List
Reagent Quantity
Assay plate 1
Standard 2
Sample Diluent 1 × 20mL
Assay Diluent A 1 × 10mL
Assay Diluent B 1 × 10mL
Detection Reagent A 1 × 60μL
Detection Reagent B 1 × 120μL
Wash Buffer(25 x concentrate) 1 × 30mL
Substrate 1 × 10mL
Stop Solution 1 × 10mL
Plate sealer 5
Test principle
Human 4-HNE ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in Human 4-HNE ELISA KIT has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectro- photometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately
1000 × g. Remove serum and assay immediately or aliquot and store samples at -20