5-HETE ELISA KIT|Human

Catalog  EF006188

Packing 48T/96T

Reactivity Human

Detect Range 0.156-10ng/ml

5-HETE ELISA KIT Sensitivity< 0.094ng/ml

Application 5-HETE ELISA KIT|Human  is for detecting the concentration of Human 5-HETE in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of 5-HETE ELISA KIT|Human

The microtiter plate provided in 5-HETE ELISA KIT|Human has been pre-coated with an Human 5-HETE antibody specific to Human 5-HETE . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human 5-HETE antibody preparation specific for Human 5-HETE then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 5-HETE , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human 5-HETE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that 5-HETE ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!