Canine Diacylglycerol, DAG ELISA Kit

Starting at: $796.00

  • Model: CELI-66018d
  • 20 Units in Stock
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Canine Diacylglycerol, DAG ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Diacylglycerol, Diglyceride, DAG

Search name
Canine Diglyceride ELISA Kit,Canine Diacylglycerol, DAG ELISA Kit, Canine DAG ELISA Kit

Intended use
 
Canine Diglyceride ELISA Kit allows for the in vitro quantitative determination of
 
diacylglycerol,Diacylglycerol concentrations in serum, plasma,
 
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Canine Diglyceride ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Diacylglycerol, During the reaction, Diacylglycerol in the sample or standard competes with a fixed
amount of biotin-labeled Diacylglycerol for sites on a pre-coated Monoclonal antibody specific to Diacylglycerol. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Diacylglycerol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine Diglyceride ELISA Kit Range 0.62-40ng/ml

Canine Diglyceride ELISA Kit PDF manual download

Order Canine Diglyceride ELISA Kit by email:sales@lifescience-market.com

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