GHITM ELISA KIT|Human

Catalog  EF006861

Packing 48T/96T

Reactivity Human

Detect Range 0.313-20ng/ml

GHITM ELISA KIT Sensitivity< 0.188ng/ml

Application GHITM ELISA KIT|Human  is for detecting the concentration of Human GHITM in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of GHITM ELISA KIT|Human

The microtiter plate provided in GHITM ELISA KIT|Human has been pre-coated with an Human GHITM antibody specific to Human GHITM . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human GHITM antibody preparation specific for Human GHITM then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human GHITM , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human GHITM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that GHITM ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!