Human Carbonic anhydrase 14, CA14 ELISA KIT

Product Name:Human Carbonic anhydrase 14, CA14 ELISA KIT
Packing:96T

Catalog No.:ELI-04639h

Gene Name:CA14

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Carbonic anhydrase 14(CA14

Alternative Name:CA14,Carbonic anhydrase 14(CA14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Carbonic anhydrase 14, CA14 ELISA KIT allows for the in vitro quantitative determination of Carbonic anhydrase 14(CA14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Carbonic anhydrase 14, CA14 ELISA KIT has been pre-coated with an Carbonic anhydrase 14(CA14 antibody specific to Carbonic anhydrase 14(CA14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carbonic anhydrase 14(CA14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbonic anhydrase 14(CA14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carbonic anhydrase 14(CA14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!