Human Contactin- 1, CNTN1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Contactin-1,Glycoprotein gp135,Neural cell surface protein F3, CNTN1,GP135; contactin-1; glycoprotein gP135; neural cell surface protein F3; contactin 1

Search name

Human Contactin-1 ELISA KIT ,Human Glycoprotein gp135 ELISA KIT ,Human Neural cell surface protein F3 ELISA KIT ,Human CNTN1 ELISA KIT ,Human GP135 ELISA KIT ,Human contactin-1 ELISA KIT ,Human glycoprotein gP135 ELISA KIT ,Human neural cell surface protein F3 ELISA KIT ,Human contactin 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Contactin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Contactin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Contactin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Contactin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Contactin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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