Human Glutamate receptor 2, GluR2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Gria2,GluR2, GluR-2,GLURB; AMPA 2,GluA2; GluR-K2; HBGR2; AMPA-selective glutamate receptor 2; gluR-2; gluR-B; glutamate receptor 2; glutamate receptor,ionotropic

Search name

Human Gria2 ELISA KIT ,Human GluR2 ELISA KIT ,Human GluR-2 ELISA KIT ,Human GLURB ELISA KIT ,Human AMPA 2 ELISA KIT ,Human GluA2 ELISA KIT ,Human GluR-K2 ELISA KIT ,Human HBGR2 ELISA KIT ,Human AMPA-selective glutamate receptor 2 ELISA KIT ,Human gluR-2 ELISA KIT ,Human gluR-B ELISA KIT ,Human glutamate receptor 2 ELISA KIT ,Human glutamate receptor ELISA KIT ,Human ionotropic ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human glutamate receptor 2,GluR-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GluR-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GluR-2 and Avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GluR-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GluR-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.