Human Histone H1.4, HIST1H1E ELISA KIT

Product Name:Human Histone H1.4, HIST1H1E ELISA KIT
Packing:96T

Catalog No.:ELI-08795h

Gene Name:HIST1H1E

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Histone H1.4(HIST1H1E

Alternative Name:HIST1H1E,Histone H1.4(HIST1H1E
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Histone H1.4, HIST1H1E ELISA KIT allows for the in vitro quantitative determination of Histone H1.4(HIST1H1E concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Histone H1.4, HIST1H1E ELISA KIT has been pre-coated with an Histone H1.4(HIST1H1E antibody specific to Histone H1.4(HIST1H1E .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone H1.4(HIST1H1E and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H1.4(HIST1H1E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone H1.4(HIST1H1E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!