Human IL6 ELISA Kit

Product Name:Human IL6 ELISA Kit
Packing:96T

Catalog No.:ELA-E0079h

Gene Name:IL6

Detect Range:1.56 pg/mL - 100 pg/mL

Sensitivity:1.04 pg/mL

Target Protein Name:Human Interleukin-6

Alternative Name:Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IL6 ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IL6 ELISA Kit has been pre-coated with an IL6 antibody specific to Human Interleukin-6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm