Human Jagged 1 Protein, JAG1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE

BEGINNING!

Synonyms

JAGGED1,AGS; AHD; AWS; CD339; HJ1; JAGL1; protein jagged-1; jagged 1

Search name

Human Jag1 ELISA KIT ,Human JAGL1 ELISA KIT ,Human JAGGED1 ELISA KIT ,Human AGS ELISA KIT ,Human AHD ELISA KIT ,Human AWS ELISA KIT ,Human CD339 ELISA KIT ,Human HJ1 ELISA KIT ,Human protein jagged-1 ELISA KIT ,Human jagged 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human protein Jagged-1, Jagged1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Jagged1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Jagged1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Jagged1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.