Human CFI ELISA Kit

Product Name:Human CFI ELISA Kit
Packing:96T

Catalog No.:ELA-E0982h

Gene Name:CFI

Detect Range:1.56-100 ng/ml

Sensitivity:0.067ng/mL

Target Protein Name:Human Complement factor I

Alternative Name:Complement factor I, C3B/C4B inactivator, CFI, IF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human CFI ELISA Kit allows for the in vitro quantitative determination of Human Complement factor I concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human CFI ELISA Kit has been pre-coated with an CFI antibody specific to Human Complement factor I .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Complement factor I and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Complement factor I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm