Human Ras- related protein Rab- 10, RAB10 ELISA KIT
Product Name:Human Ras- related protein Rab- 10, RAB10 ELISA KIT
Packing:96T
Catalog No.:ELI-35843h
Gene Name:RAB10
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ras-related protein Rab-10(RAB10
Alternative Name:RAB10,Ras-related protein Rab-10(RAB10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ras- related protein Rab- 10, RAB10 ELISA KIT allows for the in vitro quantitative determination of Ras-related protein Rab-10(RAB10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ras- related protein Rab- 10, RAB10 ELISA KIT has been pre-coated with an Ras-related protein Rab-10(RAB10 antibody specific to Ras-related protein Rab-10(RAB10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ras-related protein Rab-10(RAB10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ras-related protein Rab-10(RAB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ras-related protein Rab-10(RAB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!