Human Inhibitor of ASPP protein,IASPP ELISA KIT

Product Name: Human Inhibitor of ASPP protein,IASPP ELISA KIT
Packing:96T                                       Manual

Catalog No.:ELI-21417h

Gene Name: IASPP

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name: Inhibitor of ASPP protein

Alternative Name:PPP1R13L,RelA-associated inhibitor, IASPP, NKIP1, PPP1R13BL, RAI, Inhibitor of ASPP protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IASPP ELISA KIT allows for the in vitro quantitative determination of IASPP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IASPP ELISA KIT has been pre-coated with an IASPP antibody specific to IASPP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IASPP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IASPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of IASPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!