Human Secernin-3, SCRN3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SCRN3,SES3; secernin-3; secernin 3

Search name

Human SCRN3 ELISA KIT, Human SES3 ELISA KIT, Human secernin-3 ELISA KIT, Human secernin 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Secernin-3, SCRN3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCRN3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SCRN3 and Avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SCRN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SCRN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.