Human Spermine oxidase, SMOX ELISA KIT
Product Name:Human Spermine oxidase, SMOX ELISA KIT
Packing:96T
Catalog No.:ELI-07251h
Gene Name:SMOX
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Spermine oxidase(SMOX
Alternative Name:SMOX,Spermine oxidase(SMOX
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Spermine oxidase, SMOX ELISA KIT allows for the in vitro quantitative determination of Spermine oxidase(SMOX concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Spermine oxidase, SMOX ELISA KIT has been pre-coated with an Spermine oxidase(SMOX antibody specific to Spermine oxidase(SMOX .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Spermine oxidase(SMOX and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Spermine oxidase(SMOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Spermine oxidase(SMOX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!