AAV- U6gRNA1- U6gRNA2- TnT- Cre

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Catalog No. PVT10895
Packing 2ug

 

AAV-U6gRNA1-U6gRNA2-TnT-Cre Information


Function Mammal Editing plasmids

Promoter: U6

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli Stbl3

Culture conditions: 37 degrees centigrade

 

AAV-U6gRNA1-U6gRNA2-TnT-Cre Description

AAV-U6gRNA1-U6gRNA2-TnT-Cre is a mammalian adeno-associated virus Cre and 2 x gRNA expression plasmid.

AAV-U6gRNA1-U6gRNA2-TnT-Cre

 

AAV-U6gRNA1-U6gRNA2-TnT-Cre Sequence

LOCUS       Exported                5958 bp ds-DNA     circular SYN 26-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        278..353
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        366..606
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        633..708
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    721..1134
                     /label=gallus TnT promoter
     intron          1260..1392
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1437..1455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1467..1487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1488..2516
                     /label=Cre
     polyA_signal    2554..3030
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3070..3210
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3285..3740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4022..4126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4127..4987
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5158..5746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg actgcaggtg gaattccacc tgctcaggtt ttagagctag aaatagcaag
      301 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta
      361 cgcgtgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      421 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      481 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      541 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      601 aaggacgaaa caccggaaga gcgagctctt ctgttttaga gctagaaata gcaagttaaa
      661 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctagt
      721 gggataaaag cagtctgggc tttcacatga cagcatctgg ggctgcggca gagggtcggg
      781 tccgaagcgc tgccttatca gcgtccccag ccctgggagg tgacagctgg ctggcttgtg
      841 tcagcccctc gggcactcac gtatctccgt ccgacgggtt taaaatagca aaactctgag
      901 gccacacaat agcttgggct tatatgggct cctgtggggg aagggggagc acggaggggg
      961 ccggggccgc tgctgccaaa atagcagctc acaagtgttg cattcctctc tgggcgccgg
     1021 gcacattcct gctggctctg cccgccccgg ggtgggcgcc ggggggacct taaagcctct
     1081 gccccccaag gagcccttcc cagacagccg ccggcaccca ccgctccgtg ggacgatccc
     1141 cgaagctcta gagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
     1201 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
     1261 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1321 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1381 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat
     1441 acgactcact ataggctagc atggtgccca agaagaagag gaaagtctcc aacctgctga
     1501 ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga
     1561 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     1621 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa caacaggaaa tggttccctg
     1681 ctgaacctga ggatgtgagg gactacctcc tgtacctgca agccagaggc ctggctgtga
     1741 agaccatcca acagcacctg ggccagctca acatgctgca caggagatct ggcctgcctc
     1801 gcccttctga ctccaatgct gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg
     1861 atgctgggga gagagccaag caggccctgg cctttgaacg cactgacttt gaccaagtca
     1921 gatccctgat ggagaactct gacagatgcc aggacatcag gaacctggcc ttcctgggca
     1981 ttgcctacaa caccctgctg cgcattgccg aaattgccag aatcagagtg aaggacatct
     2041 cccgcaccga tggtgggaga atgctgatcc acattggcag gaccaagacc ctggtgtcca
     2101 cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa gctggtggag agatggatct
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