ATU6- 26- sgRNA- SK

$193.00

  • Model: PVT11217
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ATU6-26-sgRNA-SK

Catalog No. PVT11217
Packing 2ug


ATU6-26-sgRNA-SK Infomation

Plasmid size: 3760bp

Prokaryotic resistance: ampicillin Amp

Function plant Editing plasmids

 

ATU6-26-sgRNA-SK Description

ATU6-26-sgRNA-SK-vector

 

ATU6-26-sgRNA-SK Sequence

LOCUS       Exported                3760 bp ds-DNA     circular SYN 26-SEP-2017
DEFINITION  synthetic circular DNA
KEYWORDS    ATU6-26-sgRNA-SK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2017  
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        64..168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             169..1029
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1200..1789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2383..2389
                     /label=U6 terminator
     misc_RNA        complement(2390..2465)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    complement(2483..2931)
                     /label=AtU6-26 promoter
                     /note="Synthetic construct guide RNA, complete sequence
                     GenBank: KT827739.1"
     rep_origin      complement(3176..3631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ctgatgtcgc gcgcttatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
       61 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      301 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      361 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      481 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      541 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      601 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      721 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      901 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      961 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1021 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1501 gcgcagcggt cgggctgaac gggggggttc gtgcacacag cccagcttgg agcgaacgac
     1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     1981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     2041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     2101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     2161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     2221 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
     2281 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagcggcc
     2341 gccagtgtga tggatatctg cagaattgcc cttcgaaggg acaaaaaaag caccgactcg
     2401 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
     2461 aaaacaggtc ttctcgaaga cccaatcact acttcgactc tagctgtata taaactcagc
     2521 ttcgttttct tatctaagcg atgtgggact tttgaagatt gttttcaact taaatgggcc
     2581 tatataagaa atactattgt tctttcccat ataaatgggc ctgcttctct tctttcagat
     2641 tcccaggggc cttttgaaga ttatcttcat atcttaagaa tgaagatgtt ttattcaatc
     2701 aaattcttga aggttcgatg cctaatcatt ctaatcctgg gacaaactat gaaacaagat
     2761 acaaaaactc cgaatggaaa gttaaaaaga agaaaacgaa agctacggtt caagaaaatg
     2821 taagctgata aacaaaaaaa aactgtatga acgaagaaga agaaaaaaag ctaagaagaa
     2881 atgatgtatt gtgcggaagg caagtcgagt ttccgttgtt caacgaagct taagggcaat
     2941 tccagcacac tggcggccgt tactagtgga tccgagctcg gtacccaatt cgccctatag
     3001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     3181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3541 ggagcgggcg ctagggcgct ggcaagtgt
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