BE4- Gam

$160.00

  • Model: PVT10486
  • 50 Units in Stock
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BE4-Gam

Catalog No. PVT10486
Packing 2ug
Function ORF expression plasmid
Resistance Amp
Screen /
Strain Stbl3
Culture temperature 37degrees centigrade
Replicon -
Copy -
Promoter -
Induction -
Forward primer -
Reverse primer -

 

BE4-Gam Description

BE4-Gam is a rat gene mammalian cell overexpressing plasmid.

 

BE4-Gam Sequence

LOCUS       Exported                9444 bp ds-DNA     circular SYN 01-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    409..930
                     /label=Gam
     CDS             979..1662
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=rAPOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1759..5859
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5890..6138
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6169..6417
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6430..6450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6459..6476
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6505..6729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7202..7790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7961..8821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8822..8926)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9196..131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca
      421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt
      481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg
      541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc
      601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg
      661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc
      721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt
      781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa
      841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct
      901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag
      961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga
     1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag
     1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca
     1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat
     1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa
     1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac
     1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc
     1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac
     1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta
     1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt
     1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac
     1621 cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg ttcttctggt
     1681 ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagttctggt
     1741 ggttcttctg gtggttctga taaaaagtat tctattggtt tagccatcgg cactaattcc
     1801 gttggatggg ctgtcataac cgatgaatac aaagtacctt caaagaaatt taaggtgttg
     1861 gggaacacag accgtcattc gattaaaaag aatcttatcg gtgccctcct attcgatagt
     1921 ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc
     1981 aagaaccgaa tatgttactt acaagaaatt tttagcaatg agatggccaa agttgacgat
     2041 tctttctttc accgtttgga agagtccttc cttgtcgaag aggacaagaa acatgaacgg
     2101 caccccatct ttggaaacat agtagatgag gtggcatatc atgaaaagta cccaacgatt
     2161 tatcacctca gaaaaaagct agttgactca actgataaag cggacctgag gttaatctac
     2221 ttggctcttg cccatatgat aaagttccgt gggcactttc tcattgaggg tgatctaaat
     2281 ccggacaact cggatgtcga caaactgttc atccagttag tacaaaccta taatcagttg
     2341 tttgaagaga accctataaa tgcaagtggc gtggatgcga aggctattct tagcgcccgc
     2401 ctctctaaat cccgacggct agaaaacctg atcgcacaat tacccggaga gaagaaaaat
     2461 gggttgttcg gtaaccttat agcgctctca ctaggcctga caccaaattt taagtcgaac
     2521 ttcgacttag ctgaagatgc caaattgcag cttagtaagg acacgtacga tgacgatctc
     2581 gacaatctac tggcacaaat tggagatcag tatgcggact tatttttggc tgccaaaaac
     2641 cttagcgatg caatcctcct atctgacata ctgagagtta atactgagat taccaaggcg
     2701 ccgttatccg cttcaatgat caaaaggtac gatgaacatc accaagactt gacacttctc
     2761 aaggccctag tccgtcagca actgcctgag aaatataagg aaatattctt tgatcagtcg
     2821 aaaaacgggt acgcaggtta tattgacggc ggagcgagtc aagaggaatt ctacaagttt
     2881 atcaaaccca tattagagaa gatggatggg acggaagagt tgcttgtaaa actcaatcgc
     2941 gaagatctac tgcgaaagca gcggactttc gacaacggta gcattccaca tcaaatccac
     3001 ttaggcgaat tgcatgctat acttagaagg caggaggatt tttatccgtt cctcaaagac
     3061 aatcgtgaaa agattgagaa aatcctaacc tttcgcatac cttactatgt gggacccctg
     3121 gcccgaggga actctcggtt cgcatggatg acaagaaagt ccgaagaaac gattactcca
     3181 tggaattttg aggaagttgt cgataaaggt gcgtcagctc aatcgttcat cgagaggatg
     3241 accaactttg acaagaattt accgaacgaa aaagtattgc ctaagcacag tttactttac
     3301 gagtatttca cagtgtacaa tgaactcacg aaagttaagt atgtcactga gggcatgcgt
     3361 aaacccgcct ttctaagcgg agaacagaag aaagcaatag tagatctgtt attcaagacc
     3421 aaccgcaaag tgacagttaa gcaattgaaa gaggactact ttaagaaaat tgaatgcttc
     3481 gattctgtcg agatctccgg ggtagaagat cgatttaatg cgtcacttgg tacgtatcat
     3541 gacctcctaa agataattaa agataaggac ttcctggata acgaagagaa tgaagatatc
     3601 ttagaagata tagtgttgac tcttaccctc tttgaagatc gggaaatgat tgaggaaaga
     3661 ctaaaaacat acgctcacct gttcgacgat aaggttatga aacagttaaa gaggcgtcgc
     3721 tatacgggct ggggacgatt gtcgcggaaa cttatcaacg ggataagaga caagcaaagt
     3781 ggtaaaacta ttctcgattt tctaaagagc gacggcttcg ccaataggaa ctttatgcag
     3841 ctgatccatg atgactcttt aaccttcaaa gaggatatac aaaaggcaca ggtttccgga
     3901 caaggggact cattgcacga acatattgcg aatcttgctg gttcgccagc catcaaaaag
     3961 ggcatactcc agacagtcaa agtagtggat gagctagtta aggtcatggg acgtcacaaa
     4021 ccggaaaaca ttgtaatcga gatggcacgc gaaaatcaaa cgactcagaa ggggcaaaaa
     4081 aacagtcgag agcggatgaa gagaatagaa gagggtatta aagaactggg cagccagatc
     4141 ttaaaggagc atcctgtgga aaatacccaa ttgcagaacg agaaacttta cctctattac
     4201 ctacaaaatg gaagggacat gtatgttgat caggaactgg acataaaccg tttatctgat
     4261 tacgacgtcg atcacattgt accccaatcc tttttgaagg acgattcaat cgacaataaa
     4321 gtgcttacac gctcggataa gaaccgaggg aaaagtgaca atgttccaag cgaggaagtc
     4381 gtaaagaaaa tgaagaacta ttggcggcag ctcctaaatg cgaaactgat aacgcaaaga
     4441 aagttcgata acttaactaa agctgagagg ggtggcttgt ctgaacttga caaggccgga
     4501 tttattaaac gtcagctcgt ggaaacccgc caaatcacaa agcatgttgc acagatacta
     4561 gattcccgaa tgaatacgaa atacgacgag aacgataagc tgattcggga agtcaaagta
     4621 atcactttaa agtcaaaatt ggtgtcggac ttcagaaagg attttcaatt ctataaagtt
     4681 agggagataa ataactacca ccatgcgcac gacgcttatc ttaatgccgt cgtagggacc
     4741 gcactcatta agaaataccc gaagctagaa agtgagtttg tgtatggtga ttacaaagtt
     4801 tatgacgtcc gtaagatgat cgcgaaaagc gaacaggaga taggcaaggc tacagccaaa
     4861 tacttctttt attctaacat tatgaatttc tttaagacgg aaatcactct ggcaaacgga
     4921 gagatacgca aacgaccttt aattgaaacc aatggggaga caggtgaaat cgtatgggat
     4981 aagggccggg acttcgcgac ggtgagaaaa gttttgtcca tgccccaagt caacatagta
     5041 aagaaaactg aggtgcagac cggagggttt tcaaaggaat cgattcttcc aaaaaggaat
     5101 agtgataagc tcatcgctcg taaaaaggac tgggacccga aaaagtacgg tggcttcgat
     5161 agccctacag ttgcctattc tgtcctagta gtggcaaaag ttgagaaggg aaaatccaag
     5221 aaactgaagt cagtcaaaga attattgggg ataacgatta tggagcgctc gtcttttgaa
     5281 aagaacccca tcgacttcct tgaggcgaaa ggttacaagg aagtaaaaaa ggatctcata
     5341 attaaactac caaagtatag tctgtttgag ttagaaaatg gccgaaaacg gatgttggct
     5401 agcgccggag agcttcaaaa ggggaacgaa ctcgcactac cgtctaaata cgtgaatttc
     5461 ctgtatttag cgtcccatta cgagaagttg aaaggttcac ctgaagataa cgaacagaag
     5521 caactttttg ttgagcagca caaacattat ctcgacgaaa tcatagagca aatttcggaa
     5581 ttcagtaaga gagtcatcct agctgatgcc aatctggaca aagtattaag cgcatacaac
     5641 aagcacaggg ataaacccat acgtgagcag gcggaaaata ttatccattt gtttactctt
     5701 accaacctcg gcgctccagc cgcattcaag tattttgaca caacgataga tcgcaaacga
     5761 tacacttcta ccaaggaggt gctagacgcg acactgattc accaatccat cacgggatta
     5821 tatgaaactc ggatagattt gtcacagctt gggggtgact ctggtggttc tggaggatct
     5881 ggtggttcta ctaatctgtc agatattatt gaaaaggaga ccggtaagca actggttatc
     5941 caggaatcca tcctcatgct cccagaggag gtggaagaag tcattgggaa caagccggaa
 
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