Fop- Flash Plasmid

$216.00

  • Model: PVT1302
  • 50 Units in Stock
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Fop-Flash

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Fop-Flash,Plasmid Fop-Flash,Fop-Flash vector

 

Fop-Flash Information

Promoter: TK

Replicon: pUC ori

Terminator: SV40 poly (A) signal

Plasmid classification: mammalian cells, signal pathway reporting vectors

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: mammalian cells

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence

 

Fop-Flash Sequence

LOCUS       Exported                5529 bp ds-DNA     circular SYN 24-APR-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        590..652
                     /note="Mini-TK promoter"
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             681..2333
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /note="luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          2492..2557
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             2687..2707
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    3132..3266
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3710..4298)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4469..5329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5330..5434)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agggggtaag gccaaagggg
      421 gtaaggccaa agggggcccc ctttggcctt accccctttg gccttacccc ctttggcctt
      481 acctgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc ttgtcattgg
      541 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
      601 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
      661 acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
      721 tctatcctct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
      781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
      841 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
      901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
      961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     1021 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
     1081 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
     1141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     1201 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
     1261 tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
     1321 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     1381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     1441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
     1501 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
     1561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     1621 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
     1681 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
     1741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     1801 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
     1861 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     1921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     1981 tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat
     2041 tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
     2101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     2161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     2221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     2281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa
     2341 ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc caccgcggtg
     2401 gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     2701 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     2761 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgc
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