GW1- PercevalHR

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  • Model: PVT10538
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GW1-PercevalHR


Catalog No. PVT10538
Packing 2ug

 

GW1-PercevalHR Information


Function Other source plasmids

Promoter: hCMV IE4 promoter

Replicon: pUC ori

Terminator: SV40 poly (A) signal

Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.

Plasmid size: 7010bp

Prokaryotic resistance: ampicillin Amp (100 g/ml)

Screening marker: neomycin Neo/G418

Clone: DH5a Escherichia coli

Culture conditions: 37 C, aerobic, LB

Expression host: mammalian cells such as 293T

Culture conditions: 37 C, 5%CO2

Induction mode: no need to induce

 

GW1-PercevalHR Description

GW1-PercevalHR  drives PercevalHR gene expression through hCMV IE4 promoter.

 

GW1-PercevalHR Sequence

LOCUS       Exported                7010 bp ds-DNA    circular SYN 14-Aug-2017
KEYWORDS    GW1-PercevalHR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  TITLE     Direct Submission
  JOURNAL   Exported 2017-08-14
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             189..1928
                     /codon_start=1
                     /note="PercevalHR"
                     /translation="MKKVESIIRPEKLEIVKKALSDAGYVGMTVSEVKGSGVQGGIFER
                     YRGRVYSAGYNSDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVL
                     LPDNHYLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFT
                     GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGL
                     QCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNGTIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKI
                     FVIPVERVVRVRTKEEGASGGGSGGGGASGMKKVEAIIRPEKLEIVKKALSDAGYVGMT
                     VSEVKGRGAGGGDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERIV
                     RVRTKEEGASGGGGGSGGASGMKKVEAIIRPEKLEIVKKALNDDGYVGMTVSEVKGRGA
                     GGGDLIPKVKIELVVKEEDVDNIIDIICENARTGNPGDGKIFVIPVERVVRPRTKEEGK
                     EAL"
     polyA_signal    2050..2184
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2610..3198)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3369..4229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5448..7010
                     /note="hCMV IE4 promoter"
     enhancer        5448..5827
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5828..6031
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6032..7010
                     /note="CMV intron"
ORIGIN
        1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
       61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc
      121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct
      181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga
      241 aggctctctc ggacgctgga tatgtgggta tgaccgtctc tgaagtcaaa ggctctggcg
      301 tccagggcgg catcttcgag aggtaccgag gaagggtgta ctctgcaggc tacaacagcg
      361 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga
      541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg
      661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      841 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct
      901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca
     1081 acggcaccat agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg
     1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg
     1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag
     1261 catctggtgg tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta
     1321 ttcgcccaga gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca
     1381 tgaccgtttc cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga
     1441 agatcgagct ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga
     1501 acgcccgcac tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg
     1561 tgcgagtacg aaccaaagaa gagggtgcct ccggcggtgg cggtggctcc ggtggcgcca
     1621 gcggcatgaa gaaggttgag gccatcatcc gcccagagaa gctggaaatc gttaagaagg
     1681 ccttaaacga cgacggctac gtcggcatga cagttagcga ggttaaaggt cgcggtgccg
     1741 gtgggggtga tctgattcct aaggttaaaa tcgagctcgt tgttaaggag gaggacgttg
     1801 acaacattat cgatatcatc tgcgaaaacg ctcgcaccgg caacccaggc gacggcaaga
     1861 ttttcgtcat tcctgttgag agagtggtgc gtccccgtac gaaggaggag ggcaaggaag
     1921 cactgtgact cgacgaattc attgatcata atcagccata ccacatttgt agaggtttta
     1981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2161 aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc atagctgttt
     2221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
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