HBV 1.3- mer WT replicon

$193.00

  • Model: PVT10528
  • 50 Units in Stock
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HBV 1.3-mer WT replicon

Catalog No. PVT10528
Packing 2ug

 

HBV 1.3-mer WT replicon Information


Function Other source plasmids

Replicon: ori

Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.

Plasmid size: 6821bp

Prokaryotic resistance: ampicillin Amp (100 u g/ml)

Cloned strains of Escherichia coli, DH5 A and other Escherichia coli

Culture conditions: 37 centigrade, aerobic, LB

 

HBV 1.3-mer WT replicon Reference

DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse transcription by incorporation into nucleocapsids.

 

HBV 1.3-mer WT replicon Sequence

LOCUS       Exported                6821 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
 
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             843..1688
                     /codon_start=1
                     /product="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
                     /label=HBsAg (pre-S2/S)
                     /note="hepatitis B surface antigen"
                     /translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
                     FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
                     GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
                     LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
                     ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
                     "
     promoter        complement(2866..2884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2903..2919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2927..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2951..2981)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2996..3017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3305..3893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4064..4924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4925..5029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5503..5519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5527..5545
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ccggagacta ctgttgttag acgacgaggc aggtccccta gaagaagaac tccctcgcct
       61 cgcagacgaa ggtctcaatc gccgcgtcgc agaagatctc aatctcggga atctcaatgt
      121 tagtattcct tggactcata aggtggggaa ctttactggg ctttattctt ctactgtacc
      181 tgtctttaat cctcattgga aaacaccatc ttttcctaat atacatttac accaagacat
      241 tatcaaaaaa tgtgaacagt ttgtaggccc actcacagtt aatgagaaaa gaagattgca
      301 attgattatg cctgccaggt tttatccaaa ggttaccaaa tatttaccat tggataaggg
      361 tattaaacct tattatccag aacatctagt taatcattac ttccaaacta gacactattt
      421 acacactcta tggaaggcgg gtatattata taagagagaa acaacacata gcgcctcatt
      481 ttgtgggtca ccatattctt gggaacaaga tctacagcat ggggcagaat ctttccacca
      541 gcaatcctct gggattcttt cccgaccacc agttggatcc agccttcaga gcaaacaccg
      601 caaatccaga ttgggacttc aatcccaaca aggacacctg gccagacgcc aacaaggtag
      661 gagctggagc attcgggctg ggtttcaccc caccgcacgg aggccttttg gggtggagcc
      721 ctcaggctca gggcatacta caaactttgc cagcaaatcc gcctcctgcc tccaccaatc
      781 gccagtcagg aaggcagcct accccgctgt ctccaccttt gagaaacact catcctcagg
      841 ccatgcagtg gaattccaca accttccacc aaactctgca agatcccaga gtgagaggcc
      901 tgtatttccc tgctggtggc tccagttcag gaacagtaaa ccctgttctg actactgcct
      961 ctcccttatc gtcaatcttc tcgaggattg gggaccctgc gctgaacatg gagaacatca
     1021 catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa
     1081 tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt ctagggggaa
     1141 ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca ccaacctctt
     1201 gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc atcttcctct
     1261 tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa ggtatgttgc
     1321 ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc cggacctgca
     1381 tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg
     1441 gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc ctatgggagt
     1501 gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag tggttcgtag
     1561 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
     1621 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtat
     1681 acatttaaac cctaacaaaa caaagagatg gggttactct ctaaatttta tgggttatgt
     1741 cattggatgt tatgggtcct tgccacaaga acacatcata caaaaaatca aagaatgttt
     1801 tagaaaactt cctattaaca ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct
     1861 tttgggtttt gctgcccctt ttacacaatg tggttatcct gcgttgatgc ctttgtatgc
     1921 atgtattcaa tctaagcagg ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa
     1981 acaatacctg aacctttacc ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc
     2041 tgacgcaacc cccactggct ggggcttggt catgggccat cagcgcatgc gtggaacctt
     2101 ttcggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg ctcgcagcag
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