• Model: PVT10983
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Catalog No. PVT10983
Packing 2ug


lentiSAMv2 Information

Function Mammal Editing plasmids

Prokaryotic resistance: ampicillin Amp

Screening marker: bleomycin Zeo, magnaporin Blast

Cloned strain: Escherichia coli Stbl3

Culture conditions: 37 degrees centigrade


lentiSAMv2 Description

LentiSAMv2 is a mammalian CAS9 and gRNA expression plasmid


lentiSAMv2 Sequence

LOCUS       Exported               13500 bp ds-DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..13500
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        118..447
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      298..433
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        495..542
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             561..935
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="confers resistance to bleomycin, phleomycin, and 
     polyA_signal    1065..1186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1637..2225)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(2396..3256)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(3257..3361)
                     /label=AmpR promoter
     enhancer        3627..4006
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4008..4206
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
     LTR             4224..4404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4451..4576
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5069..5302
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
     misc_feature    5829..5946
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5996..6236
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        6282..6300
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
     misc_RNA        6352..6370
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
     promoter        6457..6668
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
     CDS             6685..6732
                     /product="bipartite nuclear localization signal from 
                     /label=nucleoplasmin NLS
     CDS             6733..10833
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
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