mAmetrine-N1
Catalog No. PVT10774
Packing 2ug
mAmetrine-N1 Information
Function Mammalian fluorescent plasmid
Resistance /
Screen /
Strain /
Culture temperature
mAmetrine-N1 Multiple cloning site
mAmetrine-N1 Sequence
LOCUS Exported 4732 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4732)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-18
FEATURES Location/Qualifiers
source 1..4732
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="CMV enhancer;human cytomegalovirus immediate early
enhancer"
promoter 365..568
/label=CMV promoter
/note="CMV promoter;human cytomegalovirus (CMV) immediate
early promoter"
misc_feature 591..671
/label=MCS
/note="MCS;multiple cloning site"
CDS 679..1398
/codon_start=1
/product="yellow fluorescent protein with a large Stokes
shift, excitable with violet light (Ai et al., 2008)"
/label=mAmetrine
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
EFVTAAGITPGMDELYK"
polyA_signal 1520..1641
/label=SV40 poly(A) signal
/note="SV40 poly(A) signal;SV40 polyadenylation signal"
rep_origin complement(1648..2103)
/direction=LEFT
/label=f1 ori
/note="f1 ori;f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2130..2234
/gene="bla"
/label=AmpR promoter
/note="AmpR promoter"
promoter 2236..2593
/label=SV40 promoter
/note="SV40 promoter;SV40 enhancer and early promoter"
rep_origin 2444..2579
/label=SV40 ori
/note="SV40 ori;SV40 origin of replication"
CDS 2628..3422
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="NeoR/KanR;confers resistance to neomycin, kanamycin,
and G418 (Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3654..3701
/label=HSV TK poly(A) signal
/note="HSV TK poly(A) signal;herpesvirus thymidine kinase
polyadenylation signal"
rep_origin 4030..4618
/direction=RIGHT
/label=ori
/note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc
781 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcacctc cggcaagctg
841 cccgtgccct ggcccaccct cgtgaccacc ctgtcttacg gcgtgcagtg cttcgcccgc
901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
961 caggagcgca ccatctcctt caaggacgac ggcagctaca ggacccgcgc cgaggtgaag
1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1081 ggcaacatcc tggggcacaa gctggagtac aacatgaacg tgtgggacgc gtatatcacg
1141 gccgacaagc agaagaacgg catcaaagcg aacttcaaga tcgagcacaa cgtcgaggac
1201 ggcggcgtgc agctcgccga cgcgtaccag cagaacaccc ccatcggcga cggctccgtg
1261 ctgctgcctg acaaccacta cctgagcttc cagagcaagc tgttcaaaga ccccaacgag
1321 cagcgcgatc acatggtcct gctggagttc gttaccgccg ccgggatcac tcccggcatg
1381 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt
1441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
1501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
1621 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
1681 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
1741 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
1801 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
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