pacAd5 9.2- 100

$228.00

  • Model: PVT11033
  • 50 Units in Stock
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pacAd5 9.2-100


Catalog No. PVT11033
Packing 2ug
Function /
Resistance Amp
Screen /
Strain Stbl3
Culture temperature LB/37degrees centigrade
Terminator: SV40 poly (A) signal
Plasmid Size: 34947bp

 

pacAd5 9.2-100 Description

PacAd5 9.2-100 is an adenovirus skeleton plasmid used to produce adenovirus.

Recombinant adenoviruses have tremendous potential in both research and therapeutic applications.There are numerous advantages they provide when introducing genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e.does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. Two methods have traditionally been used to generate recombinant adenoviruses. The first involves homologous recombination of a shuttle vector containing gene of interest and an adenoviral backbone plasmid vector (restricted in E1/E3) in an adenovirus packaging cell line. The isolation of recombinant adenovirus by this method involves performing multiple plaque isolations to avoid wild-type virus and is extremely laborious and time consuming. The second method, pAdEasy system, employs the homologous recombination machinery in E. coli, a recombinant adenovirus is produced by a doublerecombination event between cotransformed adenoviral backbone plasmid vector and a shuttle vector carrying the gene of interest. For the pAdEasy method, the system is high fidelity, but inefficient and requires the screening of many bacterial colonies. This results in a significant time commitment even before transfection of recombinant DNA into E1-expressing cells such as HEK293 cells.

 

pacAd5 9.2-100 Reference

RAPAd Universal Adenoviral Expression System.

 

pacAd5 9.2-100 Sequence

LOCUS       Exported               34947 bp ds-DNA     circular SYN 05-NOV-2017
DEFINITION  synthetic circular DNA
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 34947)
             
FEATURES             Location/Qualifiers
     source          1..34947
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    34..168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    169..32952
                     /label=AD
     misc_feature    4172..4275
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     repeat_region   32850..32952
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(33143..33731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(33882..34742)
                     /label=Amp
     promoter        complement(34743..34847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaattaagc tagagcggcc gccaccgcgg ggagatccag acatgataag atacattgat
       61 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      121 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      181 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      241 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg
      301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      421 cagccatgag gtcgactcta gtccccgcgg tggcagatct ggaaggtgct gaggtacgat
      481 gagacccgca ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct
      541 gtgatgctgg atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc
      601 gctgagtttg gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc
      661 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca
      721 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg
      781 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat
      841 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg
      901 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt
      961 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc
     1021 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat
     1081 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc
     1141 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag
     1201 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg
     1261 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg
     1321 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca
     1381 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta
     1441 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca
     1501 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt
     1561 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc
     1621 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg
     1681 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg
     1741 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag
     1801 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca
     1861 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt
     1921 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg
     1981 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta
     2041 ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag
     2101 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc
     2161 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc
     2221 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt
     2281 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc
     2341 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct
     2401 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct
     2461 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg
     2521 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga
     2581 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg
     2641 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa
     2701 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca
     2761 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga
     2821 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt
     2881 ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct
     2941 cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg
     3001 ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa
     3061 gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt
     3121 gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct
     3181 cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg
     3241 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     3301 ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt
     3361 tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg
     3421 agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca
     3481 cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca
     3541 ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc
     3601 cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta
     3661 gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca
     3721 ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc
     3781 gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga
     3841 gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa
     3901 gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt
     3961 gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga
     4021 ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga
     4081 agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct
     4141 tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga
     4201 tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt
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