pacAd5 9.2- 100

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  • Model: PVT11033
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pacAd5 9.2-100


Catalog No. PVT11033
Packing 2ug
Function /
Resistance Amp
Screen /
Strain Stbl3
Culture temperature LB/37degrees centigrade
Terminator: SV40 poly (A) signal
Plasmid Size: 34947bp

 

pacAd5 9.2-100 Description

PacAd5 9.2-100 is an adenovirus skeleton plasmid used to produce adenovirus.

Recombinant adenoviruses have tremendous potential in both research and therapeutic applications.There are numerous advantages they provide when introducing genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e.does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. Two methods have traditionally been used to generate recombinant adenoviruses. The first involves homologous recombination of a shuttle vector containing gene of interest and an adenoviral backbone plasmid vector (restricted in E1/E3) in an adenovirus packaging cell line. The isolation of recombinant adenovirus by this method involves performing multiple plaque isolations to avoid wild-type virus and is extremely laborious and time consuming. The second method, pAdEasy system, employs the homologous recombination machinery in E. coli, a recombinant adenovirus is produced by a doublerecombination event between cotransformed adenoviral backbone plasmid vector and a shuttle vector carrying the gene of interest. For the pAdEasy method, the system is high fidelity, but inefficient and requires the screening of many bacterial colonies. This results in a significant time commitment even before transfection of recombinant DNA into E1-expressing cells such as HEK293 cells.

 

pacAd5 9.2-100 Reference

RAPAd Universal Adenoviral Expression System.

 

pacAd5 9.2-100 Sequence

LOCUS       Exported               34947 bp ds-DNA     circular SYN 05-NOV-2017

DEFINITION  synthetic circular DNA

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 34947)

FEATURES             Location/Qualifiers

     source          1..34947

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     polyA_signal    34..168

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     misc_feature    169..32952

                     /label=AD

     misc_feature    4172..4275

                     /label=Ad SA

                     /note="adenovirus major late transcript splice acceptor 

                     (Friedrich and Soriano, 1991)"

     repeat_region   32850..32952

                     /label=ITR

                     /note="inverted terminal repeat of human adenovirus 

                     serotype 5"

     rep_origin      complement(33143..33731)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     misc_feature    complement(33882..34742)

                     /label=Amp

     promoter        complement(34743..34847)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 ttaattaagc tagagcggcc gccaccgcgg ggagatccag acatgataag atacattgat

       61 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt

      121 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat

      181 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa

      241 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg

      301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg

      361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg

      421 cagccatgag gtcgactcta gtccccgcgg tggcagatct ggaaggtgct gaggtacgat

      481 gagacccgca ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct

      541 gtgatgctgg atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc

      601 gctgagtttg gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc

      661 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca

      721 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg

      781 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat

      841 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg

      901 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt

      961 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc

     1021 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat

     1081 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc

     1141 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag

     1201 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg

     1261 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg

     1321 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca

     1381 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta

     1441 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca

     1501 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt

     1561 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc

     1621 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg

     1681 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg

     1741 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag

     1801 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca

     1861 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt

     1921 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg

     1981 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta

     2041 ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag

     2101 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc

     2161 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc

     2221 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt

     2281 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc

     2341 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct

     2401 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct

     2461 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg

     2521 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga

     2581 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg

     2641 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa

     2701 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca

     2761 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga

     2821 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt

     2881 ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct

     2941 cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg

     3001 ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa

     3061 gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt

     3121 gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct

     3181 cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg

     3241 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct

     3301 ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt

     3361 tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg

     3421 agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca

     3481 cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca

     3541 ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc

     3601 cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta

     3661 gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca

     3721 ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc

     3781 gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga

     3841 gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa

     3901 gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt

     3961 gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga

     4021 ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga

     4081 agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct

     4141 tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga

     4201 tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt

     4261 cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta

     4321 gcatgtagaa ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt

     4381 atgcctgcgc ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga

     4441 ctttgaggta ctggtatttg aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa

     4501 agtccgtgcg ctttttggaa cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta

     4561 tctttcccgc gcgaggcata aagttgcgtg tgatgcggaa gggtcccggc acctcggaac

     4621 ggttgttaat tacctgggcg gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca

     4681 caatgtaaag ttccaagaag cgcgggatgc ccttgatgga aggcaatttt ttaagttcct

     4741 cgtaggtgag ctcttcaggg gagctgagcc cgtgctctga aagggcccag tctgcaagat

     4801 gagggttgga agcgacgaat gagctccaca ggtcacgggc cattagcatt tgcaggtggt

     4861 cgcgaaaggt cctaaactgg cgacctatgg ccattttttc tggggtgatg cagtagaagg

     4921 taagcgggtc ttgttcccag cggtcccatc caaggttcgc ggctaggtct cgcgcggcag

     4981 tcactagagg ctcatctccg ccgaacttca tgaccagcat gaagggcacg agctgcttcc

     5041 caaaggcccc catccaagta taggtctcta catcgtaggt gacaaagaga cgctcggtgc

     5101 gaggatgcga gccgatcggg aagaactgga tctcccgcca ccaattggag gagtggctat

     5161 tgatgtggtg aaagtagaag tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa

     5221 aacgtgcgca gtactggcag cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac

     5281 gaccgcgcac aaggaagcag agtgggaatt tgagcccctc gcctggcggg tttggctggt

     5341 ggtcttctac ttcggctgct tgtccttgac cgtctggctg ctcgagggga gttacggtgg

     5401 atcggaccac cacgccgcgc gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct

     5461 tgatgacaac atcgcgcaga tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt

     5521 caggcgggag ctcctgcagg tttacctcgc atagacgggt cagggcgcgg gctagatcca

     5581 ggtgatacct aatttccagg ggctggttgg tggcggcgtc gatggcttgc aagaggccgc

     5641 atccccgcgg cgcgactacg gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg

     5701 atgatgcatc taaaagcggt gacgcgggcg agcccccgga ggtagggggg gctccggacc

     5761 cgccgggaga gggggcaggg gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc

     5821 gcgtaggttg ctggcgaacg cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg

     5881 cgtgaagacg acgggcccgg tgagcttgaa cctgaaagag agttcgacag aatcaatttc

     5941 ggtgtcgttg acggcggcct ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata

     6001 ggcgatctcg gccatgaact gctcgatctc ttcctcctgg agatctccgc gtccggctcg

     6061 ctccacggtg gcggcgaggt cgttggaaat gcgggccatg agctgcgaga aggcgttgag

     6121 gcctccctcg ttccagacgc ggctgtagac cacgccccct tcggcatcgc gggcgcgcat

     6181 gaccacctgc gcgagattga gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg

     6241 ctgaaagagg tagttgaggg tggtggcggt gtgttctgcc acgaagaagt acataaccca

     6301 gcgtcgcaac gtggattcgt tgatatcccc caaggcctca aggcgctcca tggcctcgta

     6361 gaagtccacg gcgaagttga aaaactggga gttgcgcgcc gacacggtta actcctcctc

     6421 cagaagacgg atgagctcgg cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc

     6481 ctcttcttct tcttcaatct cctcttccat aagggcctcc ccttcttctt cttctggcgg

     6541 cggtggggga ggggggacac ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg

     6601 ctcgatcatc tccccgcggc gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg

     6661 ggggcgcagt tggaagacgc cgcccgtcat gtcccggtta tgggttggcg gggggctgcc

     6721 atgcggcagg gatacggcgc taacgatgca tctcaacaat tgttgtgtag gtactccgcc

     6781 gccgagggac ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc

     6841 taaccagtca cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc

     6901 ggggttgttt ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg

     6961 gcggatggtc gacagaagca ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc

     7021 ggccatgccc caggcttcgt tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag

     7081 cctttctacc ggcacttctt cttctccttc ctcttgtcct gcatctcttg catctatcgc

     7141 tgcggcggcg gcggagtttg gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc

     7201 gaagcccctc atcggctgaa gcagggctag gtcggcgaca acgcgctcgg ctaatatggc

     7261 ctgctgcacc tgcgtgaggg tagactggaa gtcatccatg tccacaaagc ggtggtatgc

     7321 gcccgtgttg atggtgtaag tgcagttggc cataacggac cagttaacgg tctggtgacc

     7381 cggctgcgag agctcggtgt acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc

     7441 gttgcaagtc cgcaccaggt actggtatcc caccaaaaag tgcggcggcg gctggcggta

     7501 gaggggccag cgtagggtgg ccggggctcc gggggcgaga tcttccaaca taaggcgatg

     7561 atatccgtag atgtacctgg acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg

     7621 aaagtcgcgg acgcggttcc agatgttgcg cagcggcaaa aagtgctcca tggtcgggac

     7681 gctctggccg gtcaggcgcg cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt

     7741 aagcgggcac tcttccgtgg tctggtggat aaattcgcaa gggtatcatg gcggacgacc

     7801 ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc

     7861 gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc ttttggcttc cttccaggcg

     7921 cggcggctgc tgcgctagct tttttggcca ctggccgcgc gcagcgtaag cggttaggct

     7981 ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg agggttattt tccaagggtt

     8041 gagtcgcggg acccccggtt cgagtctcgg accggccgga ctgcggcgaa cgggggtttg

     8101 cctccccgtc atgcaagacc ccgcttgcaa attcctccgg aaacagggac gagccccttt

     8161 tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg cccccctcct cagcagcggc

     8221 aagagcaaga gcagcggcag acatgcaggg caccctcccc tcctcctacc gcgtcaggag

     8281 gggcgacatc cgcggttgac gcggcagcag atggtgatta cgaacccccg cggcgccggg

     8341 cccggcacta cctggacttg gaggagggcg agggcctggc gcggctagga gcgccctctc

     8401 ctgagcggca cccaagggtg cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc

     8461 agaacctgtt tcgcgaccgc gagggagagg agcccgagga gatgcgggat cgaaagttcc

     8521 acgcagggcg cgagctgcgg catggcctga atcgcgagcg gttgctgcgc gaggaggact

     8581 ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc

     8641 tggtaaccgc atacgagcag acggtgaacc aggagattaa ctttcaaaaa agctttaaca

     8701 accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat aggactgatg catctgtggg

     8761 actttgtaag cgcgctggag caaaacccaa atagcaagcc gctcatggcg cagctgttcc

     8821 ttatagtgca gcacagcagg gacaacgagg cattcaggga tgcgctgcta aacatagtag

     8881 agcccgaggg ccgctggctg ctcgatttga taaacatcct gcagagcata gtggtgcagg

     8941 agcgcagctt gagcctggct gacaaggtgg ccgccatcaa ctattccatg cttagcctgg

     9001 gcaagtttta cgcccgcaag atataccata ccccttacgt tcccatagac aaggaggtaa

     9061 agatcgaggg gttctacatg cgcatggcgc tgaaggtgct taccttgagc gacgacctgg

     9121 gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt gagccggcgg cgcgagctca

     9181 gcgaccgcga gctgatgcac agcctgcaaa gggccctggc tggcacgggc agcggcgata

     9241 gagaggccga gtcctacttt gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg

     9301 ccctggaggc agctggggcc ggacctgggc tggcggtggc acccgcgcgc gctggcaacg

     9361 tcggcggcgt ggaggaatat gacgaggacg atgagtacga gccagaggac ggcgagtact

     9421 aagcggtgat gtttctgatc agatgatgca agacgcaacg gacccggcgg tgcgggcggc

     9481 gctgcagagc cagccgtccg gccttaactc cacggacgac tggcgccagg tcatggaccg

     9541 catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg cagcagccgc aggccaaccg

     9601 gctctccgca attctggaag cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt

     9661 gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc cggcccgacg aggccggcct

     9721 ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa

     9781 cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca

     9841 gcagggcaac ctgggctcca tggttgcact aaacgccttc ctgagtacac agcccgccaa

     9901 cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc gcactgcggc taatggtgac

     9961 tgagacaccg caaagtgagg tgtaccagtc tgggccagac tattttttcc agaccagtag

    10021 acaaggcctg cagaccgtaa acctgagcca ggctttcaaa aacttgcagg ggctgtgggg

    10081 ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg

    10141 cctgttgctg ctgctaatag cgcccttcac ggacagtggc agcgtgtccc gggacacata

    10201 cctaggtcac ttgctgacac tgtaccgcga ggccataggt caggcgcatg tggacgagca

    10261 tactttccag gagattacaa gtgtcagccg cgcgctgggg caggaggaca cgggcagcct

    10321 ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag aagatcccct cgttgcacag

    10381 tttaaacagc gaggaggagc gcattttgcg ctacgtgcag cagagcgtga gccttaacct

    10441 gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg accgcgcgca acatggaacc

    10501 gggcatgtat gcctcaaacc ggccgtttat caaccgccta atggactact tgcatcgcgc

    10561 ggccgccgtg aaccccgagt atttcaccaa tgccatcttg aacccgcact ggctaccgcc

    10621 ccctggtttc tacaccgggg gattcgaggt gcccgagggt aacgatggat tcctctggga

    10681 cgacatagac gacagcgtgt tttccccgca accgcagacc ctgctagagt tgcaacagcg

    10741 cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc aggccaagca gcttgtccga

    10801 tctaggcgct gcggccccgc ggtcagatgc tagtagccca tttccaagct tgatagggtc

    10861 tcttaccagc actcgcacca cccgcccgcg cctgctgggc gaggaggagt acctaaacaa

    10921 ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg gcatttccca acaacgggat

    10981 agagagccta gtggacaaga tgagtagatg gaagacgtac gcgcaggagc acagggacgt

    11041 gccaggcccg cgcccgccca cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg

    11101 ggaggacgat gactcggcag acgacagcag cgtcctggat ttgggaggga gtggcaaccc

    11161 gtttgcgcac cttcgcccca ggctggggag aatgttttaa aaaaaaaaaa agcatgatgc

    11221 aaaataaaaa actcaccaag gccatggcac cgagcgttgg ttttcttgta ttccccttag

    11281 tatgcggcgc gcggcgatgt atgaggaagg tcctcctccc tcctacgaga gtgtggtgag

    11341 cgcggcgcca gtggcggcgg cgctgggttc tcccttcgat gctcccctgg acccgccgtt

    11401 tgtgcctccg cggtacctgc ggcctaccgg ggggagaaac agcatccgtt actctgagtt

    11461 ggcaccccta ttcgacacca cccgtgtgta cctggtggac aacaagtcaa cggatgtggc

    11521 atccctgaac taccagaacg accacagcaa ctttctgacc acggtcattc aaaacaatga

    11581 ctacagcccg ggggaggcaa gcacacagac catcaatctt gacgaccggt cgcactgggg

    11641 cggcgacctg aaaaccatcc tgcataccaa catgccaaat gtgaacgagt tcatgtttac

    11701 caataagttt aaggcgcggg tgatggtgtc gcgcttgcct actaaggaca atcagcgtgg

    11761 agctgaaata cagagtgggt ggagttcacg ctgcccgagg gcaactactc cgagaccatg

    11821 accatagacc ttatgaacaa cgcgatcgtg gagcactact tgaaagtggg cagacagaac

    11881 ggggttctgg aaagcgacat cggggtaaag tttgacaccc gcaacttcag actggggttt

    11941 gaccccgtca ctggtcttgt catgcctggg gtatatacaa acgaagcctt ccatccagac

    12001 atcattttgc tgccaggatg cggggtggac ttcacccaca gccgcctgag caacttgttg

    12061 ggcatccgca agcggcaacc cttccaggag ggctttagga tcacctacga tgatctggag

    12121 ggtggtaaca ttcccgcact gttggatgtg gacgcctacc aggcgagctt gaaagatgac

    12181 accgaacagg gcgggggtgg cgcaggcggc agcaacagca gtggcagcgg cgcggaagag

    12241 aactccaacg cggcagccgc ggcaatgcag ccggtggagg acatgaacga tcatgccatt

    12301 cgcggcgaca cctttgccac acgggctgag gagaagcgcg ctgaggccga agcagcggcc

    12361 gaagctgccg cccccgctgc gcaacccgag gtcgagaagc ctcagaagaa accggtgatc

    12421 aaacccctga cagaggacag caagaaacgc agttacaacc taataagcaa tgacagcacc

    12481 ttcacccagt accgcagctg gtaccttgca tacaactacg gcgaccctca gaccggaatc

    12541 cgctcatgga ccctgctttg cactcctgac gtaacctgcg gctcggagca ggtctactgg

    12601 tcgttgccag acatgatgca agaccccgtg accttccgct ccacgcgcca gatcagcaac

    12661 tttccggtgg tgggcgccga gctgttgccc gtgcactcca agagcttcta caacgaccag

    12721 gccgtctact cccaactcat ccgccagttt acctctctga cccacgtgtt caatcgcttt

    12781 cccgagaacc agattttggc gcgcccgcca gcccccacca tcaccaccgt cagtgaaaac

    12841 gttcctgctc tcacagatca cgggacgcta ccgctgcgca acagcatcgg aggagtccag

    12901 cgagtgacca ttactgacgc cagacgccgc acctgcccct acgtttacaa ggccctgggc

    12961 atagtctcgc cgcgcgtcct atcgagccgc actttttgag caagcatgtc catccttata

    13021 tcgcccagca ataacacagg ctggggcctg cgcttcccaa gcaagatgtt tggcggggcc

    13081 aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc actaccgcgc gccctggggc

    13141 gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg acgccatcga cgcggtggtg

    13201 gaggaggcgc gcaactacac gcccacgccg ccaccagtgt ccacagtgga cgcggccatt

    13261 cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga agagacggcg gaggcgcgta

    13321 gcacgtcgcc accgccgccg acccggcact gccgcccaac gcgcggcggc ggccctgctt

    13381 aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg ccgctcgaag gctggccgcg

    13441 ggtattgtca ctgtgccccc caggtccagg cgacgagcgg ccgccgcagc agccgcggcc

    13501 attagtgcta tgactcaggg tcgcaggggc aacgtgtatt gggtgcgcga ctcggttagc

    13561 ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact agattgcaag aaaaaactac

    13621 ttagactcgt actgttgtat gtatccagcg gcggcggcgc gcaacgaagc tatgtccaag

    13681 cgcaaaatca aagaagagat gctccaggtc atcgcgccgg agatctatgg ccccccgaag

    13741 aaggaagagc aggattacaa gccccgaaag ctaaagcggg tcaaaaagaa aaagaaagat

    13801 gatgatgatg aacttgacga cgaggtggaa ctgctgcacg ctaccgcgcc caggcgacgg

    13861 gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac ccggcaccac cgtagtcttt

    13921 acgcccggtg agcgctccac ccgcacctac aagcgcgtgt atgatgaggt gtacggcgac

    13981 gaggacctgc ttgagcaggc caacgagcgc ctcggggagt ttgcctacgg aaagcggcat

    14041 aaggacatgc tggcgttgcc gctggacgag ggcaacccaa cacctagcct aaagcccgta

    14101 acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag aaaagcgcgg cctaaagcgc

    14161 gagtctggtg acttggcacc caccgtgcag ctgatggtac ccaagcgcca gcgactggaa

    14221 gatgtcttgg aaaaaatgac cgtggaacct gggctggagc ccgaggtccg cgtgcggcca

    14281 atcaagcagg tggcgccggg actgggcgtg cagaccgtgg acgttcagat acccactacc

    14341 agtagcacca gtattgccac cgccacagag ggcatggaga cacaaacgtc cccggttgcc

    14401 tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg ccgcgtccaa gacctctacg

    14461 gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc cccggcgccc gcgccgttcg

    14521 aggaagtacg gcgccgccag cgcgctactg cccgaatatg ccctacatcc ttccattgcg

    14581 cctacccccg gctatcgtgg ctacacctac cgccccagaa gacgagcaac tacccgacgc

    14641 cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc agcccgtgct ggccccgatt

    14701 tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg tgctgccaac agcgcgctac

    14761 caccccagca tcgtttaaaa gccggtcttt gtggttcttg cagatatggc cctcacctgc

    14821 cgcctccgtt tcccggtgcc gggattccga ggaagaatgc accgtaggag gggcatggcc

    14881 ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc ggcggcggcg cgcgtcgcac

    14941 cgtcgcatgc gcggcggtat cctgcccctc cttattccac tgatcgccgc ggcgattggc

    15001 gccgtgcccg gaattgcatc cgtggccttg caggcgcaga gacactgatt aaaaacaagt

    15061 tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac gctcgcttgg tcctgtaact

    15121 attttgtaga atggaagaca tcaactttgc gtctctggcc ccgcgacacg gctcgcgccc

    15181 gttcatggga aactggcaag atatcggcac cagcaatatg agcggtggcg ccttcagctg

    15241 gggctcgctg tggagcggca ttaaaaattt cggttccacc gttaagaact atggcagcaa

    15301 ggcctggaac agcagcacag gccagatgct gagggataag ttgaaagagc aaaatttcca

    15361 acaaaaggtg gtagatggcc tggcctctgg cattagcggg gtggtggacc tggccaacca

    15421 ggcagtgcaa aataagatta acagtaagct tgatccccgc cctcccgtag aggagcctcc

    15481 accggccgtg gagacagtgt ctccagaggg gcgtggcgaa aagcgtccgc gccccgacag

    15541 ggaagaaact ctggtgacgc aaatagacga gcctccctcg tacgaggagg cactaaagca

    15601 aggcctgccc accacccgtc ccatcgcgcc catggctacc ggagtgctgg gccagcacac

    15661 acccgtaacg ctggacctgc ctccccccgc cgacacccag cagaaacctg tgctgccagg

    15721 cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc ctgcgccgcg ccgccagcgg

    15781 tccgcgatcg ttgcggcccg tagccagtgg caactggcaa agcacactga acagcatcgt

    15841 gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc tgatagctaa cgtgtcgtat

    15901 gtgtgtcatg tatgcgtcca tgtcgccgcc agaggagctg ctgagccgcc gcgcgcccgc

    15961 tttccaagat ggctacccct tcgatgatgc cgcagtggtc ttacatgcac atctcgggcc

    16021 aggacgcctc ggagtacctg agccccgggc tggtgcagtt tgcccgcgcc accgagacgt

    16081 acttcagcct gaataacaag tttagaaacc ccacggtggc gcctacgcac gacgtgacca

    16141 cagaccggtc ccagcgtttg acgctgcggt tcatccctgt ggaccgtgag gatactgcgt

    16201 actcgtacaa ggcgcggttc accctagctg tgggtgataa ccgtgtgctg gacatggctt

    16261 ccacgtactt tgacatccgc ggcgtgctgg acaggggccc tacttttaag ccctactctg

    16321 gcactgccta caacgccctg gctcccaagg gtgccccaaa tccttgcgaa tgggatgaag

    16381 ctgctactgc tcttgaaata aacctagaag aagaggacga tgacaacgaa gacgaagtag

    16441 acgagcaagc tgagcagcaa aaaactcacg tatttgggca ggcgccttat tctggtataa

    16501 atattacaaa ggagggtatt caaataggtg tcgaaggtca aacacctaaa tatgccgata

    16561 aaacatttca acctgaacct caaataggag aatctcagtg gtacgaaaca gaaattaatc

    16621 atgcagctgg gagagtccta aaaaagacta ccccaatgaa accatgttac ggttcatatg

    16681 caaaacccac aaatgaaaat ggagggcaag gcattcttgt aaagcaacaa aatggaaagc

    16741 tagaaagtca agtggaaatg caatttttct caactactga ggcagccgca ggcaatggtg

    16801 ataacttgac tcctaaagtg gtattgtaca gtgaagatgt agatatagaa accccagaca

    16861 ctcatatttc ttacatgccc actattaagg aaggtaactc acgagaacta atgggccaac

    16921 aatctatgcc caacaggcct aattacattg cttttaggga caattttatt ggtctaatgt

    16981 attacaacag cacgggtaat atgggtgttc tggcgggcca agcatcgcag ttgaatgctg

    17041 ttgtagattt gcaagacaga aacacagagc tttcatacca gcttttgctt gattccattg

    17101 gtgatagaac caggtacttt tctatgtgga atcaggctgt tgacagctat gatccagatg

    17161 ttagaattat tgaaaatcat ggaactgaag atgaacttcc aaattactgc tttccactgg

    17221 gaggtgtgat taatacagag actcttacca aggtaaaacc taaaacaggt caggaaaatg

    17281 gatgggaaaa agatgctaca gaattttcag ataaaaatga aataagagtt ggaaataatt

    17341 ttgccatgga aatcaatcta aatgccaacc tgtggagaaa tttcctgtac tccaacatag

    17401 cgctgtattt gcccgacaag ctaaagtaca gtccttccaa cgtaaaaatt tctgataacc

    17461 caaacaccta cgactacatg aacaagcgag tggtggctcc cgggctagtg gactgctaca

    17521 ttaaccttgg agcacgctgg tcccttgact atatggacaa cgtcaaccca tttaaccacc

    17581 accgcaatgc tggcctgcgc taccgctcaa tgttgctggg caatggtcgc tatgtgccct

    17641 tccacatcca ggtgcctcag aagttctttg ccattaaaaa cctccttctc ctgccgggct

    17701 catacaccta cgagtggaac ttcaggaagg atgttaacat ggttctgcag agctccctag

    17761 gaaatgacct aagggttgac ggagccagca ttaagtttga tagcatttgc ctttacgcca

    17821 ccttcttccc catggcccac aacaccgcct ccacgcttga ggccatgctt agaaacgaca

    17881 ccaacgacca gtcctttaac gactatctct ccgccgccaa catgctctac cctatacccg

    17941 ccaacgctac caacgtgccc atatccatcc cctcccgcaa ctgggcggct ttccgcggct

    18001 gggccttcac gcgccttaag actaaggaaa ccccatcact gggctcgggc tacgaccctt

    18061 attacaccta ctctggctct ataccctacc tagatggaac cttttacctc aaccacacct

    18121 ttaagaaggt ggccattacc tttgactctt ctgtcagctg gcctggcaat gaccgcctgc

    18181 ttacccccaa cgagtttgaa attaagcgct cagttgacgg ggagggttac aacgttgccc

    18241 agtgtaacat gaccaaagac tggttcctgg tacaaatgct agctaactat aacattggct

    18301 accagggctt ctatatccca gagagctaca aggaccgcat gtactccttc tttagaaact

    18361 tccagcccat gagccgtcag gtggtggatg atactaaata caaggactac caacaggtgg

    18421 gcatcctaca ccaacacaac aactctggat ttgttggcta ccttgccccc accatgcgcg

    18481 aaggacaggc ctaccctgct aacttcccct atccgcttat aggcaagacc gcagttgaca

    18541 gcattaccca gaaaaagttt ctttgcgatc gcaccctttg gcgcatccca ttctccagta

    18601 actttatgtc catgggcgca ctcacagacc tgggccaaaa ccttctctac gccaactccg

    18661 cccacgcgct agacatgact tttgaggtgg atcccatgga cgagcccacc cttctttatg

    18721 ttttgtttga agtctttgac gtggtccgtg tgcaccagcc gcaccgcggc gtcatcgaaa

    18781 ccgtgtacct gcgcacgccc ttctcggccg gcaacgccac aacataaaga agcaagcaac

    18841 atcaacaaca gctgccgcca tgggctccag tgagcaggaa ctgaaagcca ttgtcaaaga

    18901 tcttggttgt gggccatatt ttttgggcac ctatgacaag cgctttccag gctttgtttc

    18961 tccacacaag ctcgcctgcg ccatagtcaa tacggccggt cgcgagactg ggggcgtaca

    19021 ctggatggcc tttgcctgga acccgcactc aaaaacatgc tacctctttg agccctttgg

    19081 cttttctgac cagcgactca agcaggttta ccagtttgag tacgagtcac tcctgcgccg

    19141 tagcgccatt gcttcttccc ccgaccgctg tataacgctg gaaaagtcca cccaaagcgt

    19201 acaggggccc aactcggccg cctgtggact attctgctgc atgtttctcc acgcctttgc

    19261 caactggccc caaactccca tggatcacaa ccccaccatg aaccttatta ccggggtacc

    19321 caactccatg ctcaacagtc cccaggtaca gcccaccctg cgtcgcaacc aggaacagct

    19381 ctacagcttc ctggagcgcc actcgcccta cttccgcagc cacagtgcgc agattaggag

    19441 cgccacttct ttttgtcact tgaaaaacat gtaaaaataa tgtactagag acactttcaa

    19501 taaaggcaaa tgcttttatt tgtacactct cgggtgatta tttaccccca cccttgccgt

    19561 ctgcgccgtt taaaaatcaa aggggttctg ccgcgcatcg ctatgcgcca ctggcaggga

    19621 cacgttgcga tactggtgtt tagtgctcca cttaaactca ggcacaacca tccgcggcag

    19681 ctcggtgaag ttttcactcc acaggctgcg caccatcacc aacgcgttta gcaggtcggg

    19741 cgccgatatc ttgaagtcgc agttggggcc tccgccctgc gcgcgcgagt tgcgatacac

    19801 agggttgcag cactggaaca ctatcagcgc cgggtggtgc acgctggcca gcacgctctt

    19861 gtcggagatc agatccgcgt ccaggtcctc cgcgttgctc agggcgaacg gagtcaactt

    19921 tggtagctgc cttcccaaaa agggcgcgtg cccaggcttt gagttgcact cgcaccgtag

    19981 tggcatcaaa aggtgaccgt gcccggtctg ggcgttagga tacagcgcct gcataaaagc

    20041 cttgatctgc ttaaaagcca cctgagcctt tgcgccttca gagaagaaca tgccgcaaga

    20101 cttgccggaa aactgattgg ccggacaggc cgcgtcgtgc acgcagcacc ttgcgtcggt

    20161 gttggagatc tgcaccacat ttcggcccca ccggttcttc acgatcttgg ccttgctaga

    20221 ctgctccttc agcgcgcgct gcccgttttc gctcgtcaca tccatttcaa tcacgtgctc

    20281 cttatttatc ataatgcttc cgtgtagaca cttaagctcg ccttcgatct cagcgcagcg

    20341 gtgcagccac aacgcgcagc ccgtgggctc gtgatgcttg taggtcacct ctgcaaacga

    20401 ctgcaggtac gcctgcagga atcgccccat catcgtcaca aaggtcttgt tgctggtgaa

    20461 ggtcagctgc aacccgcggt gctcctcgtt cagccaggtc ttgcatacgg ccgccagagc

    20521 ttccacttgg tcaggcagta gtttgaagtt cgcctttaga tcgttatcca cgtggtactt

    20581 gtccatcagc gcgcgcgcag cctccatgcc cttctcccac gcagacacga tcggcacact

    20641 cagcgggttc atcaccgtaa tttcactttc cgcttcgctg ggctcttcct cttcctcttg

    20701 cgtccgcata ccacgcgcca ctgggtcgtc ttcattcagc cgccgcactg tgcgcttacc

    20761 tcctttgcca tgcttgatta gcaccggtgg gttgctgaaa cccaccattt gtagcgccac

    20821 atcttctctt tcttcctcgc tgtccacgat tacctctggt gatggcgggc gctcgggctt

    20881 gggagaaggg cgcttctttt tcttcttggg cgcaatggcc aaatccgccg ccgaggtcga

    20941 tggccgcggg ctgggtgtgc gcggcaccag cgcgtcttgt gatgagtctt cctcgtcctc

    21001 ggactcgata cgccgcctca tccgcttttt tgggggcgcc cggggaggcg gcggcgacgg

    21061 ggacggggac gacacgtcct ccatggttgg gggacgtcgc gccgcaccgc gtccgcgctc

    21121 gggggtggtt tcgcgctgct cctcttcccg actggccatt tccttctcct ataggcagaa

    21181 aaagatcatg gagtcagtcg agaagaagga cagcctaacc gccccctctg agttcgccac

    21241 caccgcctcc accgatgccg ccaacgcgcc taccaccttc cccgtcgagg cacccccgct

    21301 tgaggaggag gaagtgatta tcgagcagga cccaggtttt gtaagcgaag acgacgagga

    21361 ccgctcagta ccaacagagg ataaaaagca agaccaggac aacgcagagg caaacgagga

    21421 acaagtcggg cggggggacg aaaggcatgg cgactaccta gatgtgggag acgacgtgct

    21481 gttgaagcat ctgcagcgcc agtgcgccat tatctgcgac gcgttgcaag agcgcagcga

    21541 tgtgcccctc gccatagcgg atgtcagcct tgcctacgaa cgccacctat tctcaccgcg

    21601 cgtacccccc aaacgccaag aaaacggcac atgcgagccc aacccgcgcc tcaacttcta

    21661 ccccgtattt gccgtgccag aggtgcttgc cacctatcac atctttttcc aaaactgcaa

    21721 gataccccta tcctgccgtg ccaaccgcag ccgagcggac aagcagctgg ccttgcggca

    21781 gggcgctgtc atacctgata tcgcctcgct caacgaagtg ccaaaaatct ttgagggtct

    21841 tggacgcgac gagaagcgcg cggcaaacgc tctgcaacag gaaaacagcg aaaatgaaag

    21901 tcactctgga gtgttggtgg aactcgaggg tgacaacgcg cgcctagccg tactaaaacg

    21961 cagcatcgag gtcacccact ttgcctaccc ggcacttaac ctacccccca aggtcatgag

    22021 cacagtcatg agtgagctga tcgtgcgccg tgcgcagccc ctggagaggg atgcaaattt

    22081 gcaagaacaa acagaggagg gcctacccgc agttggcgac gagcagctag cgcgctggct

    22141 tcaaacgcgc gagcctgccg acttggagga gcgacgcaaa ctaatgatgg ccgcagtgct

    22201 cgttaccgtg gagcttgagt gcatgcagcg gttctttgct gacccggaga tgcagcgcaa

    22261 gctagaggaa acattgcact acacctttcg acagggctac gtacgccagg cctgcaagat

    22321 ctccaacgtg gagctctgca acctggtctc ctaccttgga attttgcacg aaaaccgcct

    22381 tgggcaaaac gtgcttcatt ccacgctcaa gggcgaggcg cgccgcgact acgtccgcga

    22441 ctgcgtttac ttatttctat gctacacctg gcagacggcc atgggcgttt ggcagcagtg

    22501 cttggaggag tgcaacctca aggagctgca gaaactgcta aagcaaaact tgaaggacct

    22561 atggacggcc ttcaacgagc gctccgtggc cgcgcacctg gcggacatca ttttccccga

    22621 acgcctgctt aaaaccctgc aacagggtct gccagacttc accagtcaaa gcatgttgca

    22681 gaactttagg aactttatcc tagagcgctc aggaatcttg cccgccacct gctgtgcact

    22741 tcctagcgac tttgtgccca ttaagtaccg cgaatgccct ccgccgcttt ggggccactg

    22801 ctaccttctg cagctagcca actaccttgc ctaccactct gacataatgg aagacgtgag

    22861 cggtgacggt ctactggagt gtcactgtcg ctgcaaccta tgcaccccgc accgctccct

    22921 ggtttgcaat tcgcagctgc ttaacgaaag tcaaattatc ggtacctttg agctgcaggg

    22981 tccctcgcct gacgaaaagt ccgcggctcc ggggttgaaa ctcactccgg ggctgtggac

    23041 gtcggcttac cttcgcaaat ttgtacctga ggactaccac gcccacgaga ttaggttcta

    23101 cgaagaccaa tcccgcccgc ctaatgcgga gcttaccgcc tgcgtcatta cccagggcca

    23161 cattcttggc caattgcaag ccatcaacaa agcccgccaa gagtttctgc tacgaaaggg

    23221 acggggggtt tacttggacc cccagtccgg cgaggagctc aacccaatcc ccccgccgcc

    23281 gcagccctat cagcagcagc cgcgggccct tgcttcccag gatggcaccc aaaaagaagc

    23341 tgcagctgcc gccgccaccc acggacgagg aggaatactg ggacagtcag gcagaggagg

    23401 ttttggacga ggaggaggag gacatgatgg aagactggga gagcctagac gaggaagctt

    23461 ccgaggtcga agaggtgtca gacgaaacac cgtcaccctc ggtcgcattc ccctcgccgg

    23521 cgccccagaa atcggcaacc ggttccagca tggctacaac ctccgctcct caggcgccgc

    23581 cggcactgcc cgttcgccga cccaaccgta gatgggacac cactggaacc agggccggta

    23641 agtccaagca gccgccgccg ttagcccaag agcaacaaca gcgccaaggc taccgctcat

    23701 ggcgcgggca caagaacgcc atagttgctt gcttgcaaga ctgtgggggc aacatctcct

    23761 tcgcccgccg ctttcttctc taccatcacg gcgtggcctt cccccgtaac atcctgcatt

    23821 actaccgtca tctctacagc ccatactgca ccggcggcag cggcagcaac agcagcggcc

    23881 acacagaagc aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg

    23941 gcggcagcag caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc

    24001 gagcttagaa acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa

    24061 gaacaagagc tgaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat

    24121 cacaaaagcg aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa

    24181 tactgcgcgc tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa

    24241 ctacgtcatc tccagcggcc acacccggcg ccagcacctg ttgtcagcgc cattatgagc

    24301 aaggaaattc ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga

    24361 gctgcccaag actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc

    24421 cgggtcaacg gaatacgcgc ccaccgaaac cgaattctcc tggaacaggc ggctattacc

    24481 accacacctc gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa

    24541 agtcccgctc ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact

    24601 aactcagggg cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt

    24661 ataactcacc tgacaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc

    24721 tcgcttggtc tccgtccgga cgggacattt cagatcggcg gcgccggccg ctcttcattc

    24781 acgcctcgtc aggcaatcct aactctgcag acctcgtcct ctgagccgcg ctctggaggc

    24841 attggaactc tgcaatttat tgaggagttt gtgccatcgg tctactttaa ccccttctcg

    24901 ggacctcccg gccactatcc ggatcaattt attcctaact ttgacgcggt aaaggactcg

    24961 gcggacggct acgactgaat gttaagtgga gaggcagagc aactgcgcct gaaacacctg

    25021 gtccactgtc gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa

    25081 ttgcccgagg atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag

    25141 cttgcccgta gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg

    25201 ggaccctgtg ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctt

    25261 tgttgccatc tctgtgctga gtataataaa tacagaaatt aaaatatact ggggctccta

    25321 tcgccatcct gtaaacgcca ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc

    25381 tggtactttt aacatctctc cctctgtgat ttacaacagt ttcaacccag acggagtgag

    25441 tctacgagag aacctctccg agctcagcta ctccatcaga aaaaacacca ccctccttac

    25501 ctgccgggaa cgtacgagtg cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa

    25561 accagacttt ttccggacag acctcaataa ctctgtttac cagaacagga ggtgagctta

    25621 gaaaaccctt agggtattag gccaaaggcg cagctactgt ggggtttatg aacaattcaa

    25681 gcaactctac gggctattct aattcaggtt tctctagggt tggggttatt ctctgtcttg

    25741 tgattctctt tattcttata ctaacgcttc tctgcctaag gctcgccgcc tgctgtgtgc

    25801 acatttgcat ttattgtcag ctttttaaac gctggggtcg ccacccaaga tgattaggta

    25861 cataatccta ggtttactca cccttgcgtc agcccacggt accacccaaa aggtggattt

    25921 taaggagcca gcctgtaatg ttacattcgc agctgaagct aatgagtgca ccactcttat

    25981 aaaatgcacc acagaacatg aaaagctgct tattcgccac aaaaacaaaa ttggcaagta

    26041 tgctgtttat gctatttggc agccaggtga cactacagag tataatgtta cagttttcca

    26101 gggtaaaagt cataaaactt ttatgtatac ttttccattt tatgaaatgt gcgacattac

    26161 catgtacatg agcaaacagt ataagttgtg gcccccacaa aattgtgtgg aaaacactgg

    26221 cactttctgc tgcactgcta tgctaattac agtgctcgct ttggtctgta ccctactcta

    26281 tattaaatac aaaagcagac gcagctttat tgaggaaaag aaaatgcctt aatttactaa

    26341 gttacaaagc taatgtcacc actaactgct ttactcgctg cttgcaaaac aaattcaaaa

    26401 agttagcatt ataattagaa taggatttaa accccccggt catttcctgc tcaataccat

    26461 tcccctgaac aattgactct atgtgggata tgctccagcg ctacaacctt gaagtcaggc

    26521 ttcctggatg tcagcatctg actttggcca gcacctgtcc cgcggatttg ttccagtcca

    26581 actacagcga cccaccctaa cagagatgac caacacaacc aacgcggccg ccgctaccgg

    26641 acttacatct accacaaata caccccaagt ttctgccttt gtcaataact gggataactt

    26701 gggcatgtgg tggttctcca tagcgcttat gtttgtatgc cttattatta tgtggctcat

    26761 ctgctgccta aagcgcaaac gcgcccgacc acccatctat agtcccatca ttgtgctaca

    26821 cccaaacaat gatggaatcc atagattgga cggactgaaa cacatgttct tttctcttac

    26881 agtatgatta aatgagacat gattcctcga gtttttatat tactgaccct tgttgcgctt

    26941 ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg aagtagactg cattccagcc

    27001 ttcacagtct atttgcttta cggatttgtc accctcacgc tcatctgcag cctcatcact

    27061 gtggtcatcg cctttatcca gtgcattgac tgggtctgtg tgcgctttgc atatctcagc

    27121 tgctgccatg ttgtgttgct accatgttgt tttcatgtgt tgctgccatg ctcttgtcgc

    27181 cttagatctc tctttatgta gtgttgtggt gtctctcttg tcgtgatgtg tgttttgtcc

    27241 tatatatttt aatttttaat ccaaacccct gtccccgcag gaggcctttg gccttctggt

    27301 aggccgtcat tgaaaactga cttaactcgt taaattaaaa aaatgtaaaa aataatggtt

    27361 gagactcagc ccaacatcgg cagatgaggt ggattgagac tcagcccaac atcggcagat

    27421 gaggtggatt gagactcaac cccaacattg gcagatgagg tgaattagat gaggtggatt

    27481 gagactcatg agggtggtat gagggcccga cgtccacagg tgggagttgt gctttacagt

    27541 ccaacgtgca ggacgcttgg catttgccag agaacaccaa gattggcaaa ttcgcaactg

    27601 gcgccctgtg ctcttcacag acggaaaaat gaccaaaatc tgattatttt tgtaaaacgg

    27661 aaaccgaatg tccgacaaag ttcatttgat gacttcccgg taggtctggc cctgccgctg

    27721 ggcccgacgc cgtccgggaa ttttacaaac gatttcggac gtctagcatt cactcacctt

    27781 gtcaaggacc tgaggatctc tgcaccctta ttaagaccct gtgcggtctc aaagatctta

    27841 ttccctttaa ctaataaaaa aaaataataa agcatcactt acttaaaatc agttagcaaa

    27901 tttctgtcca gtttattcag cagcacctcc ttgccctcct cccagctctg gtattgcagc

    27961 ttcctcctgg ctgcaaactt tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc

    28021 tgtccatccg cacccactat cttcatgttg ttgcagatga agcgcgcaag accgtctgaa

    28081 gataccttca accccgtgta tccatatgac acggaaaccg gtcctccaac tgtgcctttt

    28141 cttactcctc cctttgtatc ccccaatggg tttcaagaga gtccccctgg ggtactctct

    28201 ttgcgcctat ccgaacctct agttacctcc aatggcatgc ttgcgctcaa aatgggcaac

    28261 ggcctctctc tggacgaggc cggcaacctt acctcccaaa atgtaaccac tgtgagccca

    28321 cctctcaaaa aaaccaagtc aaacataaac ctggaaatat ctgcacccct cacagttacc

    28381 tcagaagccc taactgtggc tgccgccgca cctctaatgg tcgcgggcaa cacactcacc

    28441 atgcaatcac aggccccgct aaccgtgcac gactccaaac ttagcattgc cacccaagga

    28501 cccctcacag tgtcagaagg aaagctagcc ctgcaaacat caggccccct caccaccacc

    28561 gatagcagta cccttactat cactgcctca ccccctctaa ctactgccac tggtagcttg

    28621 ggcattgact tgaaagagcc catttataca caaaatggaa aactaggact aaagtacggg

    28681 gctcctttgc atgtaacaga cgacctaaac actttgaccg tagcaactgg tccaggtgtg

    28741 actattaata atacttcctt gcaaactaaa gttactggag ccttgggttt tgattcacaa

    28801 ggcaatatgc aacttaatgt agcaggagga ctaaggattg attctcaaaa cagacgcctt

    28861 atacttgatg ttagttatcc gtttgatgct caaaaccaac taaatctaag actaggacag

    28921 ggccctcttt ttataaactc agcccacaac ttggatatta actacaacaa aggcctttac

    28981 ttgtttacag cttcaaacaa ttccaaaaag cttgaggtta acctaagcac tgccaagggg

    29041 ttgatgtttg acgctacagc catagccatt aatgcaggag atgggcttga atttggttca

    29101 cctaatgcac caaacacaaa tcccctcaaa acaaaaattg gccatggcct agaatttgat

    29161 tcaaacaagg ctatggttcc taaactagga actggcctta gttttgacag cacaggtgcc

    29221 attacagtag gaaacaaaaa taatgataag ctaactttgt ggaccacacc agctccatct

    29281 cctaactgta gactaaatgc agagaaagat gctaaactca ctttggtctt aacaaaatgt

    29341 ggcagtcaaa tacttgctac agtttcagtt ttggctgtta aaggcagttt ggctccaata

    29401 tctggaacag ttcaaagtgc tcatcttatt ataagatttg acgaaaatgg agtgctacta

    29461 aacaattcct tcctggaccc agaatattgg aactttagaa atggagatct tactgaaggc

    29521 acagcctata caaacgctgt tggatttatg cctaacctat cagcttatcc aaaatctcac

    29581 ggtaaaactg ccaaaagtaa cattgtcagt caagtttact taaacggaga caaaactaaa

    29641 cctgtaacac taaccattac actaaacggt acacaggaaa caggagacac aactccaagt

    29701 gcatactcta tgtcattttc atgggactgg tctggccaca actacattaa tgaaatattt

    29761 gccacatcct cttacacttt ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg

    29821 tttcaacgtg tttatttttc aattgcagaa aatttcaagt catttttcat tcagtagtat

    29881 agccccacca ccacatagct tatacagatc accgtacctt aatcaaactc acagaaccct

    29941 agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct

    30001 ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac

    30061 ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact

    30121 taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt

    30181 aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag

    30241 gatagggcgg tggtgctgca gcagcggcga ataaactgct gccgccgccg ctccgtcctg

    30301 caggaataca acatggcagt ggtctcctca gcgatgattc gcaccgcccg cagcataagg

    30361 cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac ttaaatcagc acagtaactg

    30421 cagcacagca ccacaatatt gttcaaaatc ccacagtgca aggcgctgta tccaaagctc

    30481 atggcgggga ccacagaacc cacgtggcca tcataccaca agcgcaggta gattaagtgg

    30541 cgacccctca taaacacgct ggacataaac attacctctt ttggcatgtt gtaattcacc

    30601 acctcccggt accatataaa cctctgatta aacatggcgc catccaccac catcctaaac

    30661 cagctggcca aaacctgccc gccggctata cactgcaggg aaccgggact ggaacaatga

    30721 cagtggagag cccaggactc gtaaccatgg atcatcatgc tcgtcatgat atcaatgttg

    30781 gcacaacaca ggcacacgtg catacacttc ctcaggatta caagctcctc ccgcgttaga

    30841 accatatccc agggaacaac ccattcctga atcagcgtaa atcccacact gcagggaaga

    30901 cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac attcgggcag cagcggatga

    30961 tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag gtagacgatc cctactgtac

    31021 ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg tcatgccaaa tggaacgccg

    31081 gacgtagtca tatttcctga agcaaaacca ggtgcgggcg tgacaaacag atctgcgtct

    31141 ccggtctcgc cgcttagatc gctctgtgta gtagttgtag tatatccact ctctcaaagc

    31201 atccaggcgc cccctggctt cgggttctat gtaaactcct tcatgcgccg ctgccctgat

    31261 aacatccacc accgcagaat aagccacacc cagccaacct acacattcgt tctgcgagtc

    31321 acacacggga ggagcgggaa gagctggaag aaccatgttt ttttttttat tccaaaagat

    31381 tatccaaaac ctcaaaatga agatctatta agtgaacgcg ctcccctccg gtggcgtggt

    31441 caaactctac agccaaagaa cagataatgg catttgtaag atgttgcaca atggcttcca

    31501 aaaggcaaac ggccctcacg tccaagtgga cgtaaaggct aaacccttca gggtgaatct

    31561 cctctataaa cattccagca ccttcaacca tgcccaaata attctcatct cgccaccttc

    31621 tcaatatatc tctaagcaaa tcccgaatat taagtccggc cattgtaaaa atctgctcca

    31681 gagcgccctc caccttcagc ctcaagcagc gaatcatgat tgcaaaaatt caggttcctc

    31741 acagacctgt ataagattca aaagcggaac attaacaaaa ataccgcgat cccgtaggtc

    31801 ccttcgcagg gccagctgaa cataatcgtg caggtctgca cggaccagcg cggccacttc

    31861 cccgccagga accatgacaa aagaacccac actgattatg acacgcatac tcggagctat

    31921 gctaaccagc gtagccccga tgtaagcttg ttgcatgggc ggcgatataa aatgcaaggt

    31981 gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt agtcatgctc

    32041 atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca tttttctctc

    32101 aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa catttaaaca

    32161 ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg gactacggcc

    32221 atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac cgacagctcc

    32281 tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg attcacatcg

    32341 gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag gcgtagagac

    32401 aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa cacataaaca

    32461 cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac aacatacagc

    32521 gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa cctattaaaa

    32581 aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca

    32641 gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca

    32701 gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa

    32761 atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac tacaattccc

    32821 aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc

    32881 gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata

    32941 ttattgatga tgttaattaa gaattcggat ctgcgacgcg aggctggatg gccttcccca

    33001 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca

    33061 ggcaggtaga tgacgaccat cagggacagc ttcacggcca gcaaaaggcc aggaaccgta

    33121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa

    33181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc

    33241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt

    33301 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca

    33361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg

    33421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat

    33481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta

    33541 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct

    33601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac

    33661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa

    33721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa

    33781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt

    33841 taaatcaatc taaagtatat atgagtaaat tggtctgaca gttaccaatg cttaatcagt

    33901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

    33961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

    34021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

    34081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

    34141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca

    34201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

    34261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

    34321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

    34381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

    34441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca

    34501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

    34561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

    34621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

    34681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

    34741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga

    34801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

    34861 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg

    34921 cgtatcacga ggccctttcg tcttcaa

//

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