pActin-EGFP-beta-Actin (Homo)
Catalog No. PVT10291
Packing 2ug
Function ORF expression plasmid
Resistance Amp
Screen Neo/G418
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter beta-Actin Promoter
pActin-EGFP-beta-Actin (Homo) Description
pActin-EGFP-beta-Actin (Homo) is a human gene overexpression plasmid in mammalian cells
pActin-EGFP-beta-Actin (Homo) Sequence
LOCUS Exported 9339 bp ds-DNA circular SYN 20-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..9339
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 320..2960
/label=beta-Actin Promoter (Homo)
CDS 2971..3687
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 3703..4830
/codon_start=1
/label=beta-Actin (Homo)
/translation="MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMV
GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL
LTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTV
PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALD
FEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNS
IMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVW
IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
polyA_signal 4935..5159
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5205..5633
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5647..5977
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5828..5963
/label=SV40 ori
/note="SV40 origin of replication"
CDS 6044..6838
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 7012..7133
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(7584..8172)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8343..9203)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=Amp
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9204..9308)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatcttgg ccagctgaat ggaggcccag cggcaacaca ggtcctgcct
61 ggggatcagg tctgctctgc accccacctt gctgcctgga gccgcccacc tgacaacctc
121 tcatccctgc tctgtagatc cggtcccatc cccactgccc accccacccc cccagcactc
181 cacccagttc aacgttccac gaacccccag aaccagccct catcaacagg cagcaagaag
241 ggccccccgc ccatcgcccc acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg
301 aggcagctgg caagacgcct gcagctgaaa gatacaaggc cagggacagg acagtcccat
361 ccccaggagg cagggagtat acaggctggg gaagtttgcc cttgcgtggg gtggtgatgg
421 aggaggctca gcaagtcttc tggactgtga acctgtgtct gccactgtgt gctgggtggt
481 ggtcatcttt cccaccaggc tgtggcctct gcaaccttca agggaggagc aggtcccatt
541 ggctgagcac agccttgtac cgtgaactgg aacaagcagc ctccttcctg gccacaggtt
601 ccatgtcctt atatggactc atctttgcct attgcgacac acactcaatg aacacctact
661 acgcgctgca aagagccccg caggcctgag gtgcccccac ctcaccactc ttcctatttt
721 tgtgtaaaaa tccagcttct tgtcaccacc tccaaggagg gggaggagga ggaaggcagg
781 ttcctctagg ctgagccgaa tgcccctctg tggtcccacg ccactgatcg ctgcatgccc
841 accacctggg tacacacagt ctgtgattcc cggagcagaa cggaccctgc ccacccggtc
901 ttgtgtgcta ctcagtggac agacccaagg caagaaaggg tgacaaggac agggtcttcc
961 caggctggct ttgagttcct agcaccgccc cgcccccaat cctctgtggc acatggagtc
1021 ttggtcccca gagtccccca gcggcctcca gatggtctgg gagggcagtt cagctgtggc
1081 tgcgcatagc agacatacaa cggacggtgg gcccagaccc aggctgtgta gacccagccc
1141 ccccgccccg cagtgcctag gtcacccact aacgccccag gcctggtctt ggctgggcgt
1201 gactgttacc ctcaaaagca ggcagctcca gggtaaaagg tgccctgccc tgtagagccc
1261 accttccttc ccagggctgc ggctgggtag gtttgtagcc ttcatcacgg gccacctcca
1321 gccactggac cgctggcccc tgccctgtcc tggggagtgt ggtcctgcga cttctaagtg
1381 gccgcaagcc acctgactcc cccaacacca cactctacct ctcaagccca ggtctctccc
1441 tagtgaccca cccagcacat ttagctagct gagccccaca gccagaggtc ctcaggccct
1501 gctttcaggg cagttgctct gaagtcggca agggggagtg actgcctggc cactccatgc
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