pAdVAntage

$193.00

  • Model: PVT11020
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pAdVAntage

Catalog No. PVT11020
Packing 2ug

 

pAdVAntage Information

Function Mammal plasmids

Promoter: Lac

Replicon: pUC ori

Plasmid classification: mammalian cells, protein overexpression vectors

Plasmid size: 4392bp

Prokaryotic resistance: Amp

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: M13R:CAGGAAACAGCTATGACC

3'sequencing primers: primers designed according to sequence

 

pAdVAntage Description

The use of pAdVAntage vectors were transfected into mammalian cells can promote the initiation of translation to enhance the instantaneous protein expression in many cell types. Transfection of mammalian cells with expression vector often leads to the expression of the target protein. The double stranded RNA (dsRNA), which is produced in the transfection process, is thought to activate the dsRNA- activation inhibitor (DAI), which is one of the many kinds of enzymes involved in the host cell antiviral defense system. DAI phosphorylation of translation initiation factor eIF-2, termination of translation and generation of protein. However, with the pAdVAntage vector were transfected into DAI in the process of translation inhibition by RNA polymerase III generated adeno-associated virus type I (RNA VAI RNA) offset. RNA DAI combined with VAI to prevent its activation, so as to complete the translation and protein expression.

pAdVAntage-vector

 

pAdVAntage Sequence

 

LOCUS       Exported                4392 bp ds-DNA     circular SYN 15-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAdVAntage
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
 
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1743..1759)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1767..1783
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1791..1821)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1836..1857
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2145..2733)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2904..3764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3765..3869)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     4343..4359
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg
       61 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct
      121 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctacggcg
      181 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc
      241 ctcatcggct gaagcagggc caggtcggcg acaacgcgct cggctaatat ggcctgctgc
      301 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg
      361 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc
      421 gagagctcgg tgtacctgag acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa
      481 gtccgcacca ggtactgata tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc
      541 cagcgtaggg tggccggggc tccgggggcg aggtcttcca acataaggcg atgatatccg
      601 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg
      661 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg
      721 ccggtgaggc gtgcgcagtc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg
      781 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt
      841 tcgaaccccg gatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc
      901 caggtgtgcg acgtcagaca acgggggagc gctccttttg gcttccttcc aggcgcggcg
      961 gctgctgcgc tagctttttt ggccactggc cgcgcgcggc gtaagcggtt aggctggaaa
     1021 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc
     1081 gcaggacccc cggttcgagt ctcgggccgg ccggactgcg gcgaacgggg gtttgcctcc
     1141 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc
     1201 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag
     1261 caagagcagc ggcagacatg cagggcaccc tccccttctc ctaccgcgtc aggaggggca
     1321 acatccgcgg ctgacgcggc ggcagatggt gattacgaac ccccgcggcg ccgggcccgg
     1381 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag
     1441 cgacacccaa gggtgcagct gaagcgtgac acgcgcgagg cgtacgtgcc gcggcagaac
     1501 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca
     1561 gggcgcgagt tgcggcatgg cctgaaccgc gagcggttgc tgcgcgagga ggactttgag
     1621 cccgacgcgc ggaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta
     1681 accgcgtacg agcagacggt gaaccaggag attaactttc aaaaaagctt ggcgtaatca
     1741 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
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