pAN8- 1 Plasmid

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  • Model: PVT5302
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pAN8-1

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pAN8-1,Plasmid pAN8-1,pAN8-1 vector

 

pAN8-1 Information

Promoter: gpdA

Replicon: pUC ori

Terminator: TRPC

Plasmid classification: other host vectors; filamentous fungi expressing plasmids.

Plasmid size: 6089bp

Prokaryotic resistance: ampicillin Ampicillin

Screening markers: bleomycin Bleocin (Zeocin)

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: filamentous fungi

Culture conditions: Reference Literature

Induction mode: no induction

5'sequencing primers: Based on full sequence design

3'sequencing primers: Based on full sequence design

Note: NCBI ID:Z32751.1

Use:Filamentous fungal plasmid

 

pAN8-1 Description

pAN8-1, encoding phleomycin resistance. Protoplasts of a vigorous Phleor transformant were then retransformed using the partially homologous vector, pAN7-1 which encodes hygromycin B resistance. Retransformation of this strain to hygromycin resistance occurred at frequencies that were consistently twofold higher than with the original recipient strain. Linearised pAN7-1 DNA transformed phleomycin-resistant protoplasts at higher frequencies still.

pAN8-1-vector

 

pAN8-1 Sequence

LOCUS       Exported                6089 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAN8-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
            
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2129
                     /note="gpdA Promoter"
     misc_feature    2178..2293
                     /note="Intron"
     CDS             2302..2676
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="Zeo"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    complement(2733..3456)
                     /note="trpC terminator"
     primer_bind     complement(3464..3480)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3954..4058
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4059..4919
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5090..5678
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5966..5987
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6002..6032
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6040..6056
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6064..6080
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
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