pBacpAK9

$228.00

  • Model: PVT11322
  • 50 Units in Stock
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pBacpAK9


Catalog No. PVT11322
Packing 2ug
Function /
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
 

 

pBacpAK9 Description

Available separately or as part of the BacPAK™ Baculovirus Expression System (Cat. No. 631402). pBacPAK9 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites which facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK9 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and an ampicillin resistance gene for selection in E. coli.

 

pBacpAK9 Multiple cloning site

pBacpAK9-vector

 

 

pBacpAK9 Sequence

LOCUS       Exported                5538 bp ds-DNA     circular SYN 21-JAN-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     1337..2694
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2841..3296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3578..3682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3683..4543
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4714..5302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatcccgg
     1261 gaattcgagc tcggtaccag atcttctaga ttcgaactcg aggcctgcag ggcggccgct
     1321 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
     1381 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
     1441 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
     1501 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
     1561 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
     1621 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
     1681 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
     1741 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
     1801 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
     1861 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
     1921 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
     1981 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
     2041 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
     2101 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
     2161 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
     2221 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
     2281 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
     2341 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
     2401 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
     2461 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
     2521 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
     2581 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
     2641 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
     2701 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
     2761 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
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