pBAD24
PVT0709 2ug
pBAD24 Information
Promoter: araBAD
Replicator: ori, F1 ori
Terminator: rrnB T1 Terminator
Plasmid classification: large intestine plasmid; large intestine expression plasmid; pBAD series plasmid.
Plasmid size: 4542bp
Plasmid label: N-His, N-EK, N-Xpress
Prokaryotic resistance: ampicillin Amp (100 u g/ml)
Cloned strains of Escherichia coli, Top10 and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression of host: LMG194 and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Inducement: Arabia sugar
5'sequencing primers: PBAD30.F (AGATTAGCGGATCCTACCTG)
3'sequencing primers: PBAD30.R (CACTTCTGAGTTCGGCATGG)
pBAD24 Description
PBAD24 is derived from pBR322 vectors. The vector is designed to express and purify recombinant target protein in Escherichia coli in a dose-dependent manner. The E.coli araBAD promoter (pBAD) enhanced the soluble expression level of E.coli recombinant protein. Regulatory proteins AraC on pBAD/His and pBAD/Myc-His vectors can regulate pBad promoter.
pBAD24 Reference
Article: Involvement of hpap2 and dgkA Genes in Colistin Resistance Mediated by mcr Determinants
Author: A Gallardo, M Ugarte-Ruiz, M Hernández
Antibiotics 2020, 9(9), 531; https://doi.org/10.3390/antibiotics9090531
Received: 1 August 2020 / Revised: 19 August 2020 / Accepted: 21 August 2020 / Published: 22 August 2020
pBAD24 Sequence
LOCUS Exported File 4542 bp ds-DNA circular SYN 02-2-2015
KEYWORDS pBAD24
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4542)
TITLE Direct Submission
JOURNAL Exported 2015-2-2
FEATURES Location/Qualifiers
source 1..4542
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1120..1285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
terminator 1569..1655
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1747..1774
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1793..1884
/gene="bla"
/note="AmpR promoter"
CDS 1885..2745
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2787..3242
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 3353..3941
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
1321 tggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg
1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
1741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
1801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
1861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
1921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
1981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
2041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
2101 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
2161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
2221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
2281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
2341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
2401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
2461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
2521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
2581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
2641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
2701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
2761 tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg
2821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
2881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
2941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
3001 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
3061 gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc
3121 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
3181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
3241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
3481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
3961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
4021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
4081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
4141 ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg cgccccgaca
4201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
4261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4321 acgcgcgagg cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
4381 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
4441 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
4501 tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc
//
Caution:
Product is for research use only!
Search name
pBAD24,Plasmid pBAD24,pBAD24 vector
