pBARGPE1- Hygro Plasmid

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  • Model: PVT5306
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pBARGPE1-Hygro

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pBARGPE1-Hygro,Plasmid pBARGPE1-Hygro,pBARGPE1-Hygro vector

 

pBARGPE1-Hygro Information

Promoter: gpdA, TrpC

Replicon: pUC ori

Terminator: trpC

Plasmid classification: other host shuttle vectors; filamentous fungi expressing plasmids.

Plasmid size: 5992bp

Prokaryotic resistance: ampicillin Ampicillin

Screening markers: hygromycin Hygromycin

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: filamentous fungi

Induction mode: no induction

5'sequencing primers: Based on full sequence design

3'sequencing primers: Based on full sequence design

 

pBARGPE1-Hygro Description

PBARGPE1-Hygro is a filamentous fungus - Escherichia coli shuttle expression vector. The expression of exogenous target gene can be activated by gpdA promoter, and it can be transformed into filamentous fungi to be screened by hygromycin Hygromycin.

pBARGPE1-Hygro-vector

 

pBARGPE1-Hygro Sequence

LOCUS       Exported                5992 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBARGPE1-Hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     18..34
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..97
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(147..831)
                     /note="gpdA Promoter"
     misc_feature    1046..1186
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1372..1960)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2131..2991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2992..3096)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3202..3303
                     /gene="S. cerevisiae TRP1"
                     /note="TRP1 promoter"
     misc_feature    3394..3779
                     /note="TrpC Promotor"
     CDS             3780..4805
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    5197..5230
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    5308..5341
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    5735..5965
                     /note="trpC terminator"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 cccgggggat cctctagagt cgaggtcgac ggtatcgata agcttgatta aaggttcttg
      121 gatgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
      181 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
      241 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
      301 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
      361 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
      421 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
      481 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
      541 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
      601 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
      661 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
      721 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggcgcagt
      781 ggcgcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg ctttgcatac
      841 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag
      901 atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1021 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     1081 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     1201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
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