pBARGPE1- mCherry Plasmid

$216.00

  • Model: PVT5308
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pBARGPE1-mCherry

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pBARGPE1-mCherry,Plasmid pBARGPE1-mCherry,pBARGPE1-mCherry vector

 

pBARGPE1-mCherry Information

Use:Filamentous fungal plasmid

Promoter: gpdA, TrpC

Replicon: pUC ori

Terminator: trpC

Plasmid classification: other host shuttle vectors; filamentous fungi expressing plasmids.

Plasmid size: 6229bp

Plasmid label: mono red fluorescent protein mCherry

Prokaryotic resistance: ampicillin Ampicillin

Screening markers: LP Bar

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: filamentous fungi

Induction mode: no induction

5'sequencing primers: Based on full sequence design

3'sequencing primers: Based on full sequence design

 

pBARGPE1-mCherry Description

PBARGPE1-mCherry is a filamentous fungus - Escherichia coli shuttle expression vector, which can use gpdA promoter to activate the expression of foreign gene, and can be transformed into filamentous fungi to be screened by gpdA.

pBARGPE1-mCherry

 

pBARGPE1-mCherry Sequence

LOCUS       Exported                6229 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBARGPE1-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
           
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    28..258
                     /note="trpC terminator"
     protein_bind    complement(652..685)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(763..796)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(1188..1739)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     misc_feature    complement(1740..2125)
                     /note="TrpC Promotor"
     promoter        complement(2216..2317)
                     /gene="S. cerevisiae TRP1"
                     /note="TRP1 promoter"
     promoter        2423..2527
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2528..3388
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3559..4147
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4333..4473
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     misc_feature    4688..5372
                     /note="gpdA Promoter"
     primer_bind     complement(5422..5438)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5474..6184
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6196..6212)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccccgtccg gtcctgcccg tcaccgagat ccacttaacg ttactgaaat catcaaacag
       61 cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat
      121 ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag
      181 tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat
      241 acagctcatc tgcaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
      301 caattataca tttaatacgc gatagaaaac aaaatatagc gcgtgtacgc atgtaacatt
      361 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctt
      421 tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg
      481 gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg
      541 tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga
      601 taacagcaat acctgatcga ccaattccga tcatattcaa taacccttaa tataacttcg
      661 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
      721 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
      781 tgctatacga agttattagg tccctcgacc ggccgcggcg gttgtttgca agaccgagaa
      841 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
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