pBARGPE1 Plasmid

$260.00

  • Model: PVT5303
  • 50 Units in Stock
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pBARGPE1

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pBARGPE1,Plasmid pBARGPE1,pBARGPE1 vector

 

pBARGPE1 Information

Promoter: gpdA, TrpC

Replicon: pUC ori

Terminator: trpC

Plasmid classification: wide host shuttle vector; fungal overexpression plasmid.

Plasmid size: 5518bp

Prokaryotic resistance: ampicillin Ampicillin

Screening markers: LP Bar

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: filamentous fungi

Induction mode: no induction

5'sequencing primers: Based on full sequence design

3'sequencing primers: Based on full sequence design

Remarks: a single cut site is underlined in the atlas.

Use:Filamentous fungal plasmid

 

pBARGPE1 Description

PBARGPE1 is a filamentous fungus - Escherichia coli shuttle expression vector, which can use gpdA promoter to activate the expression of foreign gene, and can be transformed into filamentous fungi to be screened by gpdA.

pBARGPE1-vector

pBARGPE1 Sequence

LOCUS       Exported                5518 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBARGPE1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2015-4-17  
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
COMMENT     http://www.miaolingbio.com/
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(49..133)
                     /label=polylinker
                     /note="polylinker"
     misc_feature    complement(147..831)
                     /label=gpdA Promotor
                     /note="gpdA Promoter"
                     /note="gpdA Promotor;promoter region of the A.nidulans gpdA
                     gene"
     misc_feature    complement(1372..1960)
                     /label=pUC origin
                     /note="pUC origin"
     misc_feature    complement(2131..2991)
                     /label=AmpR
                     /note="AmpR"
                     /note="AmpR;beta-lactamase"
     misc_feature    complement(2992..3096)
                     /label=AmpR promoter
                     /note="AmpR promoter"
     misc_feature    3394..3779
                     /label=TrpC Promotor
                     /note="TrpC Promotor"
     misc_feature    3780..4331
                     /label=Bar
                     /note="Bar"
                     /note="Bar;phosphinothricin acetyltransferase"
     misc_feature    5261..5491
                     /label=trpC terminator
                     /note="trpC terminator"
                     /note="trpC terminator;derived from Aspergillus nidulans 
                     TrpC"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 cccgggggat cctctagagt cgaggtcgac ggtatcgata agcttgatta aaggttcttg
      121 gatgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
      181 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
      241 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
      301 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
      361 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
      421 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
      481 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
      541 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
      601 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
      661 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
      721 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggcgcagt
      781 ggcgcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg ctttgcatac
      841 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag
      901 atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1021 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     1081 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     1201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3121 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3181 gccctttcgt cttcaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
     3241 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
     3301 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
     3361 cagagcacag aggccgcaga atgtgctcta gactcgacag aagatgatat tgaaggagca
     3421 ctttttgggc ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc
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