pBAV1k-T5-LUX
Catalog No. PVT10531
Packing 2ug
pBAV1k- T5- LUX Information
Function Other source plasmids
Promoter: T5 promoter
Terminator: rrnB T1 Terminator
Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.
Plasmid size: 8599bp
Prokaryotic resistance: kanamycin Kan (50 mu g/ml)
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 30 centigrade, aerobic, LB
Note: low copy
pBAV1k- T5- LUX Description
Expresses the Photorhabdus luminescens lux operon in bacterial cells.The copy number of pBAV1k plasmids are not well controlled, so purification yields vary. A lacI+ E. coli strain will prevent constitutive expression of the lux operon.
pBAV1k- T5- LUX Sequence
LOCUS Exported 8599 bp ds-DNA circular SYN 15-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8599
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 26..70
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 46..62
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 78..94
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 104..115
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 122..1204
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
RBS 1216..1227
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1234..2217
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
RBS 2229..2240
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2247..3689
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
RBS 3701..3712
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 3719..4642
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
RBS 4654..4665
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 4672..5784
/codon_start=1
/gene="luxE"
/product="LuxE"
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
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