pBBR1MCS2- Tac- mCherry


  • Model: PVT11376
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Catalog No. PVT11376
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pBBR1MCS2-Tac-mCherry Information

Promoter: Tac

Replicator: pBBR1 Rep, pBBR1 oriV

Terminator: T7 Terminator

Plasmid classification: red fluorescent protein expression plasmid; wide host plasmid.

Plasmid size: 6195bp

Plasmid label: mCherry

Prokaryotic resistance: kanamycin Kanamycin

Screening markers: DH5 alpha

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: Gram-negative bacteria

5'sequencing primers: M13R:CAGGAAACAGCTATGACC

3'sequencing primers: M13F:TGTAAAACGACGGCCAGT


pBBR1MCS2-Tac-mCherry Description
Tac strong promoter activated the expression of mCherry red fluorescent protein.



pBBR1MCS2-Tac-mCherry Sequence

LOCUS       Exported                6195 bp ds-DNA     circular SYN 11-SEP-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pBBR1MCS2-Tac-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6195)
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             458..1252
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418 
     protein_bind    1657..1678
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1693..1723
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     promoter        1792..1810
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1840..1856
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
     promoter        complement(1910..1987)
                     /note="lacI promoter"
     misc_feature    2033..2061
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             2108..2818
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
     terminator      2852..2899
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
     primer_bind     complement(2941..2957)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
     promoter        complement(2990..3008)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
     CDS             complement(3741..4403)
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
     rep_origin      4404..5173
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
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