pBSFI- myr- akt1

$193.00

  • Model: PVT10429
  • 50 Units in Stock
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pBSFI-myr-akt1

Catalog No. PVT10429
Packing 2ug

 

pBSFI-myr-akt1 Information
Function DNA Cloning plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
 

pBSFI-myr-akt1 Sequence

LOCUS       Exported                4477 bp ds-DNA     circular SYN 30-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   .
  TITLE     Direct Submission
            
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    63..95
                     /label=myristolation signal
     misc_feature    96..1535
                     /label=Akt1
     CDS             1536..1562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1632..1650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1660..1676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1817..2272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2299..2403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2404..3264
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3435..4023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4409..4425
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4446..4464
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agctggccat tacggccgcg gccgcctcta gaggatctgg taccacgcgt atcgataagc
       61 ttatggggag cagcaagagc aagcccaagt ctagaatgaa cgacgtagcc attgtgaagg
      121 agggctggct gcacaaacga ggggaatata ttaaaacctg gcggccacgc tacttcctcc
      181 tcaagaacga tggcaccttt attggctaca aggaacggcc tcaggatgtg gatcagcgag
      241 agtccccact caacaacttc tcagtggcac aatgccagct gatgaagaca gagcggccaa
      301 ggcccaacac ctttatcatc cgctgcctgc agtggaccac agtcattgag cgcaccttcc
      361 atgtggaaac gcctgaggag cgggaagaat gggccaccgc cattcagact gtggccgatg
      421 gactcaagag gcaggaagaa gagacgatgg acttccgatc aggctcaccc agtgacaact
      481 caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgtgtg accatgaacg
      541 agtttgagta cctgaaacta ctgggcaagg gcacctttgg gaaagtgatt ctggtgaaag
      601 agaaggccac aggccgctac tatgccatga agatcctcaa gaaggaggtc atcgtcgcca
      661 aggatgaggt tgcccacacg cttactgaga accgtgtcct gcagaactct aggcatccct
      721 tccttacggc cctcaagtac tcattccaga cccacgaccg cctctgcttt gtcatggagt
      781 atgccaacgg gggcgagctc ttcttccacc tgtctcgaga gcgcgtgttc tccgaggacc
      841 gggcccgctt ctatggtgcg gagattgtgt ctgccctgga ctacttgcac tccgagaaga
      901 acgtggtgta ccgggacctg aagctggaga acctcatgct ggacaaggac gggcacatca
      961 agataacgga cttcgggctg tgcaaggagg ggatcaagga tggtgccact atgaagacat
     1021 tctgcggaac gccggagtac ctggcccctg aggtgctgga ggacaacgac tacggccgtg
     1081 cagtggactg gtgggggctg ggcgtggtca tgtatgagat gatgtgtggc cgcctgccct
     1141 tctacaacca ggaccacgag aagctgttcg agctgatcct catggaggag atccgcttcc
     1201 cgcgcacact cggccctgag gccaagtccc tgctctccgg gctgctcaag aaggacccta
     1261 cacagaggct cggtgggggc tctgaggatg ccaaggagat catgcagcac cggttctttg
     1321 ccaacatcgt gtggcaggat gtgtatgaga agaagctgag cccacctttc aagccccagg
     1381 tcacctctga gactgacacc aggtatttcg atgaggagtt cacagctcag atgatcacca
     1441 tcacgccgcc tgatcaagat gacagcatgg agtgtgtgga cagtgagcgg aggccgcact
     1501 tcccccagtt ctcctactca gccagtggca cagcctaccc atacgatgtt ccagattacg
     1561 cttctagatg aattcacgcg taagcttatc gatgtcgacg ggcccggccg cctcggccgt
     1621 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     1681 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     1741 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     1801 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     1861 tgttaaatca gctcattttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     1921 aaaagataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     1981 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2041 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2101 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2161 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2221 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     2281 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2341 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2401 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2461 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2521 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2581 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2641 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2701 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
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