pCAGGS- Myc

$160.00

  • Model: PVT10729
  • 50 Units in Stock
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pCAGGS-Myc


PVT10729  2ug
 

pCAGGS-Myc Information

Promoter: CAG promoter

Replicon: Ori, SV40 ori

Terminator: beta -globin poly (A) signal

Plasmid classification: lactation cell plasmid; lactation expression plasmid; pCAG series plasmid.

Plasmid size: 4809bp

Plasmid tagging: N-Myc

Prokaryotic resistance: ampicillin Amp

Clone strain: Escherichia coli DH5 alpha

Culture conditions: 37 C, aerobic, LB

Expression host: mammalian cells such as 293T

 

pCAGGS-Myc Descrption

pCAGGS-Myc is a mammalian eukaryotic expression plasmid. The CAG promoter drives the fusion expression of Myc tags and target genes.

 

 

pCAGGS-Myc Sequence

LOCUS       Exported                4809 bp ds-DNA     circular SYN 17-AUG-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pCAGGS-Myc.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4809)
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      1..10
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             10..39
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_site      169..224
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        757..952
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      803..938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    958..1092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1330..1918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2089..2949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2950..3054)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3085..3464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3466..3743
                     /label=chicken beta-actin promoter
     intron          3744..4761
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 gccaccatgg aacaaaaact catctcagaa gaggatctgg aattcgagct catcgatggt
       61 acccgggcat gcctcgagct agcagatctt aattaattaa ggatcttttt ccctctgcca
      121 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      181 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
      241 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
      301 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
      361 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
      421 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
      481 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
      541 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
      601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      661 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      721 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
      781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
      961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1081 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
     1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
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