PCAMBIA1302
Catalog No. PVT11141
Packing 2ug
PCAMBIA1302 Information
Function plant expression plasmid
Resistance Kan
Screen HygR
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pVS1V
Copy
Promoter Lac,35s
PCAMBIA1302 Description
1.High copy number in E.coli for high DNA yields2.
pVS1 replicon for high stability in Agrobacterium
3.Small size
4.Restriction sites designed for modular plasmid modifications and small but adequatepoly-linkers for introducing your DNA of interest
5. Bacterial selection with kanamycin
6.Plant selection with hygromycin B
7.Simple means to construct translational fusions to gusA reporter genes
PCAMBIA1302 Cloning site
PCAMBIA1302 Sequence
LOCUS Exported 10549 bp ds-DNA circular SYN 13-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..10549
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 17..727
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/label=mgfp5
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
CDS 734..751
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 783..1035
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 1057..1081
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 2381..3010
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 3444..4511
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 4577..4771
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 5115..5255
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(5441..6029)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6116..6910)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 7335..7359
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 7437..7611
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(7651..8676)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=Hyg
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(8744..9421)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 9612..9633
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9648..9678
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9686..9702
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 9722..9955
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 9736..9792
/label=MCS
/note="pUC18/19 multiple cloning site"
promoter 10189..10534
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
ORIGIN
1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
4021 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
4081 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
4141 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
4201 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
4261 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
4321 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
4381 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
4441 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
4501 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
4561 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
4621 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
4681 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
4741 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
4801 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
4861 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
4921 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
4981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
5041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
5101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
5161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
5221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
5881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
6121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
6181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
6241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
6301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
6361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
6421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
6481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
6541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
6601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
6661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
6721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
6781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
6841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
6901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
6961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
7021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
7081 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
7141 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
7201 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
7261 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
7321 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
7381 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
7441 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
7501 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
7561 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt
7621 gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
7681 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
7741 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
7801 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
7861 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
7921 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
7981 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
8041 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
8101 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
8161 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
8221 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
8281 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
8341 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
8401 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
8461 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
8521 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
8581 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
8641 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc
8701 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
8761 aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc
8821 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
8881 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
8941 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
9001 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
9061 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
9121 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc
9181 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
9241 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
9301 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
9361 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
9421 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
9481 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt
9541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
9601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
9661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
9721 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
9781 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
9961 cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat
10021 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct
10081 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt
10141 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
10201 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
10261 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
10321 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
10381 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
10441 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
10501 tatataagga agttcatttc atttggagag aacacggggg actcttgac
//
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