pCAMBIA1304

$260.00

  • Model: PVT3016
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pCAMBIA1304

PVT3016        2ug

 

pCAMBIA1304 Information

Promoter: CaMV 35S promoter
Replicator: pVS1 oriV, ori
Terminator: NOS
Plasmid size: 12361bp
Prokaryotic resistance: Kan
Screening markers: HygR
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expressing the host: plant
5'sequencing primers: M13F:TGTAAAACGACGGCCAGT
Use: Plant expression

 

pCAMBIA1304 Description

pCAMBIA1304 contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.The pCambia vector backbone is derived from the pPZP vectors.

pCambia1304 vector offers:

1.high copy number in E.coli for high DNA yield 
2.pVS1 replicon for high stability in Agrobacterium
3.small size 
4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest
5.bacterial selection with kanamycin 
6.plant selection with hygromycin B 7.simple means to construct translational fusions to mgfp5-gusA reporter genes.

pCAMBIA1304-polyclonal-site

 

pCAMBIA1304 Sequence

LOCUS       Exported               12361 bp ds-DNA     circular SYN 14-SEP-2016

DEFINITION  synthetic circular DNA

KEYWORDS    Untitled 4

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 12361)

  TITLE     Direct Submission

FEATURES             Location/Qualifiers

     source          1..12361

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             17..727

                     /codon_start=1

                     /gene="mgfp5"

                     /product="GFP with folding enhancement mutations"

                     /note="mgfp5"

                     /note="suitable for expression in plants due to removal of 

                     a cryptic intron"

                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF

                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN

                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN

                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF

                     VTAAGITHGMDELYK"

     CDS             734..2539

                     /codon_start=1

                     /gene="uidA (or gusA) from E. coli"

                     /product="beta-glucuronidase"

                     /note="GUS"

                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA

                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM

                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF

                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV

                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL

                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA

                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK

                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL

                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT

                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP

                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"

     CDS             2546..2563

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /note="6xHis"

                     /translation="HHHHHH"

     terminator      2595..2847

                     /note="NOS terminator"

                     /note="nopaline synthase terminator and poly(A) signal"

     misc_feature    2869..2893

                     /note="RB T-DNA repeat"

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             4193..4822

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /note="pVS1 StaA"

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     CDS             5256..6323

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /note="pVS1 RepA"

                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA

                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR

                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR

                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL

                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA

                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV

                     MRYRNLIEGEASAGS"

     rep_origin      6389..6583

                     /note="pVS1 oriV"

                     /note="origin of replication for the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

     misc_feature    6927..7067

                     /note="bom"

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(7253..7841)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7928..8722)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /note="KanR"

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     misc_feature    9147..9171

                     /note="LB T-DNA repeat"

                     /note="left border repeat from nopaline C58 T-DNA"

     polyA_signal    9249..9423

                     /note="CaMV poly(A) signal"

                     /note="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(9463..10488)

                     /codon_start=1

                     /gene="aph(4)-Ia"

                     /product="aminoglycoside phosphotransferase from E. coli"

                     /note="HygR"

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

                     K"

     promoter        complement(10556..11233)

                     /note="CaMV 35S promoter (enhanced)"

                     /note="cauliflower mosaic virus 35S promoter with a 

                     duplicated enhancer region"

     protein_bind    11424..11445

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding site"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     promoter        11460..11490

                     /note="lac promoter"

                     /note="promoter for the E. coli lac operon"

     protein_bind    11498..11514

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="lac operator"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     primer_bind     11522..11538

                     /note="M13 rev"

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     CDS             11534..11767

                     /codon_start=1

                     /gene="lacZ fragment"

                     /product="LacZ-alpha fragment of beta-galactosidase"

                     /note="lacZ-alpha"

                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR

                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"

     misc_feature    11548..11604

                     /note="MCS"

                     /note="pUC18/19 multiple cloning site"

     primer_bind     complement(11608..11624)

                     /note="M13 fwd"

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     promoter        12001..12346

                     /note="CaMV 35S promoter"

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

ORIGIN

        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt

       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga

      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc

      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga

      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag

      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg

      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat

      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa

      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt

      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc

      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga

      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact

      721 atacaaagct agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg

      781 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag

      841 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga

      901 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa

      961 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt

     1021 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga

     1081 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga

     1141 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa

     1201 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta

     1261 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg

     1321 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg

     1381 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt

     1441 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa

     1501 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa

     1561 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga

     1621 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt

     1681 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat

     1741 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt

     1801 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga

     1861 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc

     1921 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg

     1981 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc

     2041 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag

     2101 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga

     2161 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca

     2221 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta

     2281 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt

     2341 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc

     2401 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc

     2461 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc

     2521 gcagcaggga ggcaaacaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca

     2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg

     2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa

     2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat

     2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc

     2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata

     2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg

     2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg

     3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca

     3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc

     3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact

     3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc

     3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg

     3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag

     3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac

     3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc

     3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg

     3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc

     3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc

     3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa

     3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc

     3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc

     3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc

     3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg

     3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc

     4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa

     4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg

     4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt

     4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc

     4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag

     4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg

     4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg

     4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat

     4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa

     4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat

     4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat

     4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac

     4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga

     4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac

     4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg

     4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag

     4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc

     5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa

     5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg

     5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg

     5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac

     5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc

     5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc

     5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc

     5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg

     5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg

     5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg

     5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat

     5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg

     5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg

     5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca

     5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg

     5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc

     6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga

     6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag

     6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga

     6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga

     6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg

     6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag

     6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc

     6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt

     6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt

     6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca

     6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg

     6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca

     6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag

     6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg

     6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg

     6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga

     6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc

     7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt

     7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact

     7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag

     7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata

     7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc

     7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg

     7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc

     7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg

     7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc

     7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga

     7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg

     7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa

     7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg

     7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt

     7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt

     7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct

     7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga

     8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat

     8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt

     8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg

     8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat

     8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg

     8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag

     8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca

     8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt

     8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc

     8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag

     8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg

     8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat

     8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa

     8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt

     8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta

     8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc

     9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg

     9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc

     9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg

     9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta

     9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga

     9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag

     9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag

     9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg

     9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca

     9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac

     9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct

     9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc

     9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg

     9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca

     9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc

     9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc

     9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg

    10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc

    10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc

    10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc

    10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac

    10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa

    10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa

    10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc

    10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc

    10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc

    10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg

    10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga

    10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg

    10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt

    10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg

    10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc

    10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt

    10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct

    11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc

    11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt

    11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg

    11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta

    11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct

    11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa

    11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct

    11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac

    11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga

    11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga

    11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg

    11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga

    11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat

    11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt

    11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca

    11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat

    12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat

    12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg

    12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc

    12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt

    12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca

    12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga

    12361 c

//

 

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