pCas9
PVT6317 2ug
pCas9 Information
Replicon: p15A ori
Plasmid classification: Escherichia coli plasmids; Escherichia coli plasmids; large intestine Cas9 plasmids
Plasmid size: 9326bp
Prokaryotic resistance: chloramphenicol Chl (50 mu g/ml)
Cloned strains of Escherichia coli, Stbl3 and other Escherichia coli
Culture conditions: 37 centigrade, LB, aerobic
Expression host: Escherichia coli
Induction mode: no induction
5'sequencing primers: StrepPyoCas9-5UTR-F (CGGTGCCACTTTTTCAAGTTG)
3'sequencing primers: pBRrevBam (GGTGATGTCGGCGATATAGG)
Use:CRISPR-CAS26-sgRNA plasmid
pCas9 Description
pCas9 plasmid is a CRISPR/Cas9 system carrier. The replicator is p15A ori and ori. The plasmid size is 9326bp, which can be transformed into the corresponding host cells. The mass raising condition is LB, 37 C.
PCas9 can express Cas9 protein in Escherichia coli, and pCRISPR can be used for gene knockout, low copy plasmid, Bacterial expression of Cas9 nuclease, tracrRNA and crRNA.
PCas9 can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commonly used Streptococcus pyogenes Cas9 (SpCas9) can be modified to recognize alternative PAM sequences using structural information, bacterial selection-baseddirected evolution, and combinatorial design. CRISPR-Cas9 nucleases enable efficient genome editing in a wide variety of organisms and cell types Target recognition by Cas9. Site is programmed by a chimeric single guide RNA (sgRNA) that encodes a sequence complementary to a target protospacer5, but a LSO requires recognition of a short neighboring PAM the most robust. SpCas9, and widely used Cas9 to date, primarily recognizes NGG PAMs and is consequently restricted to sites that contain this motif It therefore be challenging. Can to implement genome editing applications that require precision, such as: homology-directed repair (HDR), which is most efficient when DSBs are placed within BPS of a desired 10 - 20 alteration; the introduction of variable-length insertion or deletion (indel) mutations into small size genetic elements such as microRNAs, splice sites, short open reading frames, or transcription factor binding sites by non-homologous end-joining (NHEJ); and allele-specific editing, where PAM recognition might be exploited to Differentiate alleles.
pCas9 Sequence
LOCUS Exported 9326 bp ds-DNA circular SYN 28-JUN-2017
DEFINITION synthetic circular DNA
KEYWORDS pCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9326)
TITLE Direct Submission
JOURNAL Exported Wednesday, June 28, 2017
FEATURES Location/Qualifiers
source 1..9326
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(220..322)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(848..1393)
/direction=LEFT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 1505..1533
/note="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
misc_RNA complement(1851..1929)
/note="tracrRNA"
/note="trans-activating CRISPR RNA for the Streptococcus
pyogenes CRISPR/Cas9 system"
CDS 2225..6331
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/note="Cas9"
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
misc_feature 6352..6483
/note="crRNA leader"
/note="crRNA leader sequence for the Streptococcus pyogenes
CRISPR/Cas system"
repeat_region 6484..6519
/note="DR"
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
repeat_region 6550..6585
/note="DR"
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
CDS complement(8886..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag
1741 aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag
1801 cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc
1861 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg
1921 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa
1981 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact
2041 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga
2101 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa
2161 ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc
2221 aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc
2281 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
2341 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
2401 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
2461 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
2521 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
2581 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
2641 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
2701 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
2761 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
2821 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
2881 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
2941 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
3001 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
3061 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
3121 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
3181 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
3241 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
3301 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
3361 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
3421 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
3481 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
3541 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa
3601 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga
3661 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
3721 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
3781 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt
3841 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
3901 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga
3961 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
4021 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
4081 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
4141 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
4201 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
4261 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
4321 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
4381 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
4441 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
4501 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
4561 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
4621 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
4681 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
4741 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
4801 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
4861 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
4921 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
4981 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
5041 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
5101 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
5161 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
5221 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
5281 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
5341 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
5401 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
5461 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
5521 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct
5581 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt
5641 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc
5701 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat
5761 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc
5821 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga
5881 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc
5941 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt
6001 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg
6061 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga
6121 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg
6181 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac
6241 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg
6301 cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta
6361 ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga
6421 ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac
6481 gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc tcggaagctc
6541 aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga
6601 cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc
6661 gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt
6721 taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag
6781 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
6841 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
6901 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
6961 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
7021 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
7081 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
7141 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
7201 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
7261 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
7321 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
7381 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
7441 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
7501 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
7561 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
7621 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
7681 cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc
7741 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
7801 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
7861 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
7921 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
7981 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
8041 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
8101 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
8161 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc
8221 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata
8281 ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc
8341 gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
8401 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc
8461 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc
8521 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
8581 aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta
8641 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca
8701 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
8761 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
8821 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
8881 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
8941 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
9001 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
9061 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
9121 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
9181 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
9241 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
9301 agctcaccgt ctttcattgc catacg
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
Search name
pCas9,Plasmid pCas9,pCas9 vector