pCDH-CMV-mCherry-EF1-CopGFP-Puro

Catalog No. PVT11078
Packing 2ug

 

pCDH-CMV-mCherry-EF1-CopGFP-Puro Information

Plasmid type: Lentivirus expression vector; cDNA expression vector; Double promoter vector

Cloning methods: polyclonal sites, restrictive endonucleases

Promoter: CMV

Carrier size: -

5'Sequencing Primers and Sequences: CMV-F: CGCAAATGGCGTAGGCGTG

3'sequencing primers and sequences: --

Carrier label: no

Vector resistance: ampicillin (Ampicillin)

Screening markers: GFP, Puromycin

Clone strain: E. coli cells (RecA-) recommendation: Stbl2, OmniMAX 2 T1R

Host cell (line): Common cell lines such as HeLa, HEK293, HT1080, H1299

Note: pCDH-CMV-MCS-EF1-copGFP-T2A-Puro lentiviral expression vector is a lentiviral vector based on HIV.

For cDNA expression and cloning, high efficiency transfection cells, stable cell lines were established.

The CMV promoter drives the high-level expression of the target gene, and the EF1a promoter drives the middle level expression of the reporter gene.

Stability: stable expression

Composition / inducible type: composition

Virus / non virus: lentivirus (HIV)